eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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3/2010
vol. 35
 
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abstract:

Experimental immunology
Experimental comparison of some methods to assess cytotoxicity

Monika Pieczykolan
,
Agnieszka Piechnik
,
Justyna Piłat
,
Jacek Tabarkiewicz
,
Jacek Roliński

(Centr Eur J Immunol 2010; 35 (3): 142-145)
Online publish date: 2010/10/05
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We have been witnessing an intensive growth in the medical and pharmaceutical fields in the recent years. New therapies are being introduced and elements of potential medicines are being developed, both of which require numerous trials and tests before they can be universally used. Prerequisite stage of many laboratory tests is cytotoxicity assessment. For that reason, it is vital to check reliability of such techniques. In this study, we compared ATPlite and ATPlite 1step tests based on the assessment of ATP released by cells and the measurement of chemiluminescence as well as DELFIA® EuTDA Cytotoxicity assay based on labeling of target cells with Europium and evaluating TRF. As target cells, the following cell lines were used: Jurkat, HL-60, RPMI 8226, and CCRF 913. In order to determine the linearity, we assessed values of the signals read with increasing numbers of target cells. A reliable test should be quantitative, reproducible and should ensure high sensitivity with a minimal number of cells.Test DELFIA® EuTDA Cytotoxicity has the most advantages and fulfils these requirements.
keywords:

cytotoxicity test, cell line, cytotoxicity, ATP, time resolved fluorescence, TRF

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