eISSN: 2084-9869
ISSN: 1233-9687
Polish Journal of Pathology
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1/2018
vol. 69
 
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abstract:
Original paper

Expression of growth hormone-releasing hormone receptors in apocrine adnexal tumours and apocrine glands of the skin

Bence Kővári, Lajos Kocsis, Erika Varga, Gábor Cserni

Pol J Pathol 2018; 69 (1): 48-52
Online publish date: 2018/05/07
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Encouraged by our previous finding of growth hormone-releasing hormone receptor (GHRH-R) expression in metaplastic and neoplastic apocrine breast epithelium, we examined a small series of skin adnexal tumours with various degrees of apocrine (oxyphilic) differentiation, as well as normal axillary and anogenital apocrine sweat glands, for the expression of GHRH-R.

Sections of formalin-fixed paraffin-embedded tissue blocks were immunostained for gross cystic disease fluid protein-15 (GCDFP-15) and androgen receptor (AR), to prove apocrine differentiation and correlate it with areas of GHRH-R expression.

All but one of 19 tumours with apocrine epithelium and all five benign apocrine glands stained with both anti-GHRH-R antibodies used, each labelling a different domain of the receptor. Non-apocrine areas of the tumours and four eccrine tumours without oxyphilic features did not stain, but most sebaceous glands and some eccrine glands were labelled.

Our data suggest that anti-GHRH-R antibodies highlight apocrine differentiation at extramammary sites also. Although GHRH-R seems to have a sensitivity comparable to classic apocrine markers such as AR and GCDFP-15, it seems to be inferior in specificity. The GHRH-R labelling of apocrine glands and neoplastic epithelium might also interfere with the emerging anti-GHRH targeted treatment of some malignancies acting on these receptors.
keywords:

skin adnexal tumours, sweat gland, apocrine gland, growth hormone-releasing hormone receptor, immunohistochemistry

references:
Kazakov DV, Michal M, Kacerovska D, McKee PH. Lesions with predominant apocrine and eccrine differentiation. In: Kazakov DV, Michal M, Kacerovska D, McKee PH. Cutaneous adnexal tumors. Wolters Kluwer Health/Lippincott Williams & Wilkins, Philadelphia 2012; 1-171.
Vranic S, Schmitt F, Sapino A, et al. Apocrine carcinoma of the breast: a comprehensive review. Histol Histopathol 2013; 28: 1393-1409.
Kahán Z, Arencibia JM, Csernus VJ, et al. Expression of growth hormone-releasing hormone (GHRH) messenger ribonucleic acid and the presence of biologically active GHRH in human breast, endometrial, and ovarian cancers. J Clin Endocrinol Metab 1999; 84: 582-589.
Barabutis N, Schally AV. Knocking down gene expression for growth hormone-releasing hormone inhibits proliferation of human cancer cell lines. Br J Cancer 2008; 98: 1790-1796.
Kővári B, Rusz O, Schally AV, et al. Differential immunostaining of various types of breast carcinomas for growth hormone-releasing hormone (GHRH) receptor – Apocrine epithelium and carcinomas emerging as uniformly positive. APMIS 2014; 122: 824-831.
Kővári B, Vranic S, Marchio C, et al. The expression of GHRH and its receptors in breast carcinomas with apocrine differentiation-further evidence of the presence of a GHRH pathway in these tumors. Hum Pathol 2017; 64: 164-170.
Seitz S, Rick FG, Schally AV, et al. Combination of GHRH antagonists and docetaxel shows experimental effectiveness for the treatment of triple-negative breast cancers. Oncol Rep 2013; 30: 413-418.
Perez R, Schally AV, Popovics P, et al. Antagonistic analogs of growth hormone-releasing hormone increase the efficacy of treatment of triple negative breast cancer in nude mice with doxorubicin; A preclinical study. Oncoscience 2014; 24: 665-673.
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