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Polish Journal of Pathology
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vol. 67
Original paper

Expression pattern of ISL-1, TTF-1 and PAX5 in olfactory neuroblastoma

Piotr Czapiewski
Adam Gorczyński
Johannes Haybaeck
Krzysztof Okoń
Joanna Reszeć
Wojciech Skrzypczak
Jarosław Dzierżanowski
Michał Kunc
Joanna Karczewska
Wojciech Biernat

Pol J Pathol 2016; 67 (2): 130-135
Online publish date: 2016/08/02
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Olfactory neuroblastoma (ONB), also known as esthesioneuroblastoma, was originally described in 1924 by the French physicians Berger and Luca in 1924. With its frequency of 0.4/million/year [1], it accounts for 3% of all sinonasal malignancies [2].
ONB is believed to be derived from olfactory epithelium, but its pathogenesis is largely unknown. Data on viral pathogenesis in some cases of animal ONB were not confirmed in humans [3]. ONB is frequently hormonally active and can thus result in paraneoplastic syndromes [4].
ONB presents with neuroendocrine differentiation, and primary as well as metastatic tumors can mimic other tumor entities with this differentiation pattern. Therefore, a continuous search for new markers that broaden differentiation with other neuroendocrine tumors is ongoing. Recent evidence shows that ISL-1, TTF-1 and PAX5 occur in a large percentage of neuroendocrine tumors [5, 6, 7, 8, 9]. However, the frequency of their expression and their expression pattern have not been studied in ONB.
No data are available about the functional relationship at the molecular level of TTF-1, PAX5 and ISL-1 in the literature. Also no relationship in their immunohistochemical expression has been studied despite their frequent presence in neuroendocrine tumors.
The aim of our study was to evaluate immunohistochemical expression of TTF-1, ISL-1 and PAX5 in a cohort of ONB collected from 4 large university hospitals in order to show their potential diagnostic usefulness. Additionally we assessed the potential reciprocal relationship between expression of these proteins in order to give some suggestions about the potential correlation in their biological activity and neoplastic transformation.

Material and methods


Databases of the departments and institutes of pathology in 4 large university hospitals were searched for the diagnosis of ONB or esthesioneuroblastoma. The retrieved cases were sent to the central laboratory (Medical University of Gdansk) for reevaluation. It was performed according to the criteria defined by the WHO classification of head and neck tumors.


Expression of PAX5, TTF-1 and ISL-1 was evaluated by immunohistochemistry. For TTF-1 and PAX5 ready-to-use antibodies from Dako (Denmark) were used and the staining was performed using an automated tissue stainer (Dako, Denmark). The analysis of ISL-1 (1 H9, Abcam, 1 : 1500) was performed manually.
Intensity of immunohistochemical reaction was evaluated using the h-score system. In h-score, the intensity of reaction and the percentage of positive cells were multiplied and summed up (1× n%+2×n%+3× n% = y), i.e., the score ranged from 0 to 300. Each case was evaluated for each protein by two experienced pathologists (P.C. and A.G.).


Spearman’s rank correlation test was performed using Statistica 12 in order to evaluate the correlation in expression of ISL-1, PAX5 and TTF-1.



The study group included 11 cases of ONB: 4 males and 7 females, aged 20-86 (mean: 52.3).
Basic clinicopathological characteristics of the patients are given in Table I.

Expression of TTF-1

TTF-1 was expressed in 3 cases (27.27%). Expression was nuclear and occurred in 1-27% of cells. In all cases there were at least single cells with strong immunoreactivity; the h-score of positive cases were in the range 3-45. Representative staining is shown in Fig. 1.

Expression of ISL-1

ISL-1 was expressed in 7/11 cases (63.63%). Expression was nuclear and it was observed in 15-90% of cells. The h-score of positive cases ranged between 23 and 200. Representative staining is shown in Fig. 2.

Expression of PAX5

PAX5 was expressed in 3/11 (27.27%) cases. One of them showed only single weakly positive cells (h-score: 3). The other tumors showed higher expression of PAX5 (16% and 35% of positive cells, h-score: 24 and 85, respectively). The patient with the highest level of PAX5 expression developed lymph node metastases at the primary diagnosis, and these recurred shortly afterwards with dissemination to the spine. She died 3 months after the initial diagnosis. PAX5 immunohistochemical staining is shown in Fig. 3.

Correlations in the expression of the markers

We did not observe statistically significant correlation between the expression of investigated proteins. However, taking into account the low number of cases it can be stated that there is a trend toward a moderate correlation between expression of TTF-1 and PAX5. The Spearman rank correlation test resulted in the following values for the investigated proteins:
• ISL-1 and TTF-1 ( = 0.0029; p = 0.99),
• ISL-1 and PAX5 ( = 0.0828; p = 0.81),
• TTF-1 and PAX5 ( = 0.43; p = 0.18).


PAX5 is a member of the paired box (PAX) family of genes that are proteins involved in the regulation of development and differentiation of multiple tissue types [10]. PAX5 is a transcription factor that controls B-cell development and is present on B-cells from early to late stages of B-cell differentiation [11]. Initially it was believed to be a specific marker of B-cell neoplasms, but it has also been identified in many other types of tumors with neuronal and neuroendocrine differentiation [12, 13, 14].
PAX5 is activated in the mesencephalon during murine embryogenesis [10], and knock-out of this gene results in malformations of the midbrain [15]. The role of PAX5 in the development and function of the olfactory epithelium is not known.
We showed for the first time that PAX5 is expressed in a subpopulation of ONB (3/11; 27.27%). In one of them only 3% of cells showed a weak reaction, which from a diagnostic point of view is meaningless. Two other cases revealed stronger immunoexpression in 16 and 35% of cancer cells, respectively. This observation adds ONB to the large group of neuroendocrine tumors that may show PAX5 reactivity.
The patient with the highest PAX5 expression exhibited a very aggressive clinical course of disease: lymph node metastases at the diagnosis, rapid progression with spinal involvement and death 3 months after the onset of therapy. Unfortunately, the clinical course of the remaining two PAX5-positive cases could not be retrieved from the clinical files. Therefore, it is not possible to evaluate the role of PAX5 expression regarding the clinical course and outcome. Its evaluation in an independent and larger cohort of PAX5-positive ONBs may answer this question.
TTF-1 is a marker of pulmonary and thyroid origin of tumors [16]. However, up to 40% of high-grade neuroendocrine tumors of extrapulmonary origin show expression of this transcription factor [8, 9]. In the human fetal brain, TTF-1 expression has been reported in the infundibular anlage and ependymal and subependymal cells of the third ventricle [17]. Its role in the development and function of the olfactory epithelium remains unknown.
In the primary tumors, 3/11 cases were positive, although in one case only single cells were strongly positive (h-score = 3), while the other two cases were positive in 20-27% of cells.
Some authors believe that TTF-1 expression excludes the diagnosis of ONB. However, we think that TTF-1 expression is just a marker of neuroendocrine differentiation, as it may be present in a substantial group of neuroendocrine tumors at various locations. Therefore, it does not exclude per se diagnosis of ONB. On the other hand, in none of our cases was strong, diffuse expression observed, so the differential diagnosis with the most common neuroendocrine malignancy, small cell carcinoma of the lung, which is usually strongly TTF-1 positive, should not be problematic in this setting.
ISL-1 was originally described by Karlsson et al. as a protein that binds to an insulin gene enhancer and regulates its expression [18]. Its crucial role in the development and maturation of the endocrine cells in the pancreas was confirmed [19]. Initially expression of ISL-1 was believed to be restricted to neuroendocrine tumors of pancreatic origin. However, recent data show that it can be expressed in the majority of neuroendocrine tumors of extrapancreatic origin, including Merkel cell carcinomas, pulmonary small cell neuroendocrine carcinoma, medullary thyroid carcinomas, paragangliomas/pheochromocytomas, and adrenal neuroblastomas [6].
Recently we have described the expression of ISL-1 in 44% (11/25) of medulloblastomas (Czapiewski, Pathology Research and Practice, in press). In the developing brain ISL-1 promotes striatonigral and represses striatopallidal genetic programs [20]. The role of ISL-1 in the development and function of olfactory epithelium is not known.
In a report regarding ISL-1 expression in a small group of 4 cases of ONB, only 1 case was positive [6]. In our cohort of ONB patients expression of ISL-1 was present in the majority of cases (63.63%). All of the positive cases were at least moderately immunoreactive, and in 3 cases immunoexpression was very strong (h-score 170-200).
These results increase the group of tumors with neuroendocrine differentiation and at the same time make this marker even less specific for the diagnosis of pancreatic neuroendocrine tumors than previously thought. Positive reaction with this antibody does not rule out and does not confirm the diagnosis of ONB. This can be of diagnostic value in metastases of neuroendocrine neoplasm to the sinonasal area. We do not know the expression of this marker in ONB metastases, but probably it may also be misleading under these clinical circumstances.
To sum up, ISL-1 can show a positive reaction in ONB and should be taken into consideration in the differential diagnosis of neuroendocrine tumors of the sinonasal area.
We did not observe a statistically significant correlation in expression of PAX5, ISL-1 and TTF-1 in our cohort of ONB. However, we detected a trend toward a moderate correlation between expression of PAX5 and TTF-1. The lack of significant correlation may be due small number of cases. It cannot be excluded that no relationship exists. On the other hand, this may be a real connection which could point to a common role of TTF-1 and PAX5 in biological activity and neoplastic transformation of these two proteins. However, no data confirming this dependency in neuroendocrine or any other tumor exist. This aspect requires further studies on larger ONB cohorts and in other neuroendocrine tumors.


Our results shed light on the diagnostic role of ISL-1, PAX5 and TTF-1 in ONB. Frequent ISL-1 positivity in ONB increases the spectrum of ISL-1 positive tumors with neuronal and neuroendocrine differentiation and proves that ISL-1 should not be considered as a marker of pancreatic origin in pancreatic tumors.
TTF-1 positivity does not exclude the diagnosis of ONB. PAX5 expression is infrequent in ONB, but strong reactivity can be associated with high grade tumors and very aggressive clinical course with rapid skeletal dissemination.
PAX5 and TTF-1 show a trend toward a correlation in expression which might be secondary to a yet undescribed molecular cooperation during the process of neoplastic transformation of cells.

The authors declare no conflict of interest.
This study was supported by the ST-95 grant from the Medical University of Gdansk.


1. Theilgaard SA, Buchwald C, Ingeholm P, et al. Esthesioneuroblastoma: a Danish demographic study of 40 patients registered between 1978 and 2000. Acta Otolaryngol 2003; 123: 433-439.
2. Broich G, Pagliari A, Ottaviani F. Esthesioneuroblastoma: a general review of the cases published since the discovery of the tumour in 1924. Anticancer Res 1997; 17: 2683-2706.
3. Lubojemska A, Borejko M, Czapiewski P, et al. Of mice and men: olfactory neuroblastoma among animals and humans. Vet Comp Oncol 2014; doi: 10.1111/vco.12102.
4. Kunc M, Gabrych A, Czapiewski P, Sworczak K. Paraneoplastic syndromes in olfactory neuroblastoma. Contemp Oncol (Pozn) 2015; 19: 6-16.
5. Schmitt AM, Riniker F, Anlauf M, et al. Islet 1 (Isl1) expression is a reliable marker for pancreatic endocrine tumors and their metastases. Am J Surg Pathol 2008; 32: 420-425.
6. Agaimy A, Erlenbach-Wunsch K, Konukiewitz B, et al. ISL1 expression is not restricted to pancreatic well-differentiated neuroendocrine neoplasms, but is also commonly found in well and poorly differentiated neuroendocrine neoplasms of extrapancreatic origin. Mod Pathol 2013; 26: 995-1003.
7. Torlakovic E, Slipicevic A, Robinson C, et al. Pax-5 expression in nonhematopoietic tissues. Am J Clin Pathol 2006; 126: 798-804.
8. Agoff SN, Lamps LW, Philip AT, et al. Thyroid transcription factor-1 is expressed in extrapulmonary small cell carcinomas but not in other extrapulmonary neuroendocrine tumors. Mod Pathol 2000; 13: 238-242.
9. Jones TD, Kernek KM, Yang XJ, et al. Thyroid transcription factor 1 expression in small cell carcinoma of the urinary bladder: an immunohistochemical profile of 44 cases. Hum Pathol 2005; 36: 718-723.
10. Robson EJ, He SJ, Eccles MR. A PANorama of PAX genes in cancer and development. Nat Rev Cancer 2006; 6: 52-62.
11. O’Brien P, Morin P, Jr., Ouellette RJ, Robichaud GA. The Pax-5 gene: a pluripotent regulator of B-cell differentiation and cancer disease. Cancer Res 2011; 71: 7345-7350.
12. Sica G, Vazquez MF, Altorki N, et al. PAX-5 expression in pulmonary neuroendocrine neoplasms: its usefulness in surgical and fine-needle aspiration biopsy specimens. Am J Clin Pathol 2008; 129: 556-562.
13. Song J, Li M, Tretiakova M, et al. Expression patterns of PAX5, c-Met, and paxillin in neuroendocrine tumors of the lung. Arch Pathol Lab Med 2010; 134: 1702-1705.
14. Zur Hausen A, Rennspiess D, Winnepenninckx V, et al. Early B-cell differentiation in Merkel cell carcinomas: clues to cellular ancestry. Cancer Res 2013; 73: 4982-4987.
15. Pfeffer PL, Bouchard M, Busslinger M. Pax2 and homeodomain proteins cooperatively regulate a 435 bp enhancer of the mouse Pax5 gene at the midbrain-hindbrain boundary. Development 2000; 127: 1017-1028.
16. Ordó¼ez NG. Thyroid transcription factor-1 is a marker of lung and thyroid carcinomas. Adv Anat Pathol 2000; 7: 123-127.
17. Lee EB, Tihan T, Scheithauer BW, et al. Thyroid transcription factor 1 expression in sellar tumors: a histogenetic marker? J Neuropathol Exp Neurol 2009; 68: 482-488.
18. Karlsson O, Thor S, Norberg T, et al. Insulin gene enhancer binding protein Isl-1 is a member of a novel class of proteins containing both a homeo- and a Cys-His domain. Nature 1990; 344: 879-882.
19. Du A, Hunter CS, Murray J, et al. Islet-1 is required for the maturation, proliferation, and survival of the endocrine pancreas. Diabetes 2009; 58: 2059-2069.
20. Lu KM, Evans SM, Hirano S, Liu FC. Dual role for Islet-1 in promoting striatonigral and repressing striatopallidal genetic programs to specify striatonigral cell identity. Proc Natl Acad Sci U S A 2014; 111: E168-177.

Address for correspondence

Piotr Czapiewski
Department of Pathomorphology
Medical University of Gdańsk
Dębinki 7
80-952 Gdansk, Poland
tel. +48 58 349 27 40
e-mail: czapiewskipiotr@gumed.edu.pl
Copyright: © 2016 Polish Association of Pathologists and the Polish Branch of the International Academy of Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (http://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
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