IMPROVED RAT STROKE MODEL BY INTRALUMINAL MIDDLE CEREBRAL ARTERY OCCLUSION: A SPECIAL EMPHASIS ON SURGICAL TECHNIQUE

Alex Orădan; Adina Huțanu; Emőke Horváth; Liviu Chiriac; Minodora Dobreanu

doi:10.5114/hpc.2017.70008

eISSN: 2354-0265
ISSN: 2353-6942

Health Problems of Civilization Physical activity: diseases and issues recognized by the WHO

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3/2017
vol. 11

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Review paper

IMPROVED RAT STROKE MODEL BY INTRALUMINAL MIDDLE CEREBRAL ARTERY OCCLUSION: A SPECIAL EMPHASIS ON SURGICAL TECHNIQUE

Alex Orădan

¹

,

Adina Huțanu

^{2, 3}

,

Emőke Horváth

⁴

,

Liviu Chiriac

⁵

,

Minodora Dobreanu

^{2, 3}

Laboratory animal facility – Centre for Experimental Medicine, Iuliu Hațieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania
Center for Advanced Medical and Pharmaceutical Research, University of Medicine and Pharmacy of Târgu-Mureș, Romania
Department of Laboratory Medicine, University of Medicine and Pharmacy of Târgu-Mureș, Romania
Department of Pathology, University of Medicine and Pharmacy, Târgu-Mureş, Romania
National Magnetic Resonance Center, Faculty of Physics, Babes-Bolyai University, Cluj-Napoca, Romania

Health Problems of Civilization. 2017; 11(3): 202-210

DOI: https://doi.org/10.5114/hpc.2017.70008

Online publish date: 2017/10/26

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Various rodent models have been developed for examining either focal or global cerebral ischemia so far, but the most used model is the rat middle cerebral artery occlusion by intraluminal filaments, followed by reperfusion, and 2,3,5-triphenyltetrazoliumchloride or Cresyl Violet/Nissl staining for evaluation of the region of interest. Like other surgical procedures, this one is prone to various complications (excessive bleeding, vascular or nerve lesions) and failure related to the surgical technique. We focused on detailed surgical techniques, along with data from literature, in order to reduce the complications and increase the chance of experiment success. Magnetic Resonance Imaging evaluation with 3D reconstruction of the ischemic area opens new perspectives on investigations into the ischemic brain, having the advantage of being noninvasive as an alternative to gold histological standard for measuring the infarct size, being the only one accesible in surviving subjects.

keywords:

IMPROVED RAT STROKE MODEL BY INTRALUMINAL MIDDLE CEREBRAL ARTERY OCCLUSION: A SPECIAL EMPHASIS ON SURGICAL TECHNIQUE

Alex Orădan 1 , Adina Huțanu 2, 3 , Emőke Horváth 4 , Liviu Chiriac 5 , Minodora Dobreanu 2, 3

stroke, middle cerebral artery occlusion, cerebral ischemia

Alex Orădan

¹

,

Adina Huțanu

^{2, 3}

,

Emőke Horváth

⁴

,

Liviu Chiriac

⁵

,

Minodora Dobreanu

^{2, 3}