eISSN: 2449-8238
ISSN: 2392-1099
Clinical and Experimental Hepatology
Current issue Archive Manuscripts accepted About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
SCImago Journal & Country Rank
2/2020
vol. 6
 
Share:
Share:
more
 
 
abstract:
Original paper

Induction of synthesis of matrix metalloproteinases by interleukin-6; evidence for hepatic regeneration following hemi-hepatectomy

Thamer Alghamdi
1
,
Ihab Shafek Atta
2, 3
,
Mohamed El-Refaei
4

1.
Surgery Department, Albaha Faculty of Medicine, Saudi Arabia
2.
Pathology Department, Faculty of Medicine, Al-Azhar University, Assuit, Egypt
3.
Pathology Department, Faculty of Medicine, Albaha University, Saudi Arabia
4.
Biochemistry Department, Albaha University, Faculty of Medicine, Saudi Arabia
Clin Exp HEPATOL 2020; 6, 2: 137–141
Online publish date: 2020/05/27
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Aim of the study
Interleukin-6 (IL-6) can play a role in hepatic regeneration through many mechanisms, one of which is the induction of synthesis of matrix metalloproteinases (MMPs). The aim of the study is to focus on the significance and role of MMPs in the regenerative process to reveal the correlation between IL-6 and MMPs in rats following partial hepatectomy.

Material and methods
Following hemi-hepatectomy, eighty male rats were divided into a control group and a group treated with IL-6 35 µg/100 gm body weight according to a lethality study. The blood samples were drawn from all animal groups for MMP-9 serum level assessment. For the quantitative determination of MMP-9 an enzyme-linked immunosorbent assay (ELISA) was used (Cytoimmune Science Inc., MD) through the quantitative sandwich immunosorbent assay technique. A monoclonal antibody for MMP-9 was pre-coated onto microplate standards. After washing away the unbound substances, an enzyme-linked polyclonal antibody specific for cytokine was added to the wells and color developed in proportion to the amount of total cytokine (pro and/or active) bound in the initial step. The color development was stopped and the intensity of the color was measured.

Results
The liver regeneration rate (%) was significantly higher in the group of rats treated with IL-6 (median value was 49.55% vs. 33.20%), p < 0.001. The MMPs’ serum level was significantly higher in the group of rats with resection and treatment (median value was 8.01).

Conclusions
These results give evidence for the vital role of MMPs in the process of hepatic regeneration, the level of which, in turn, has a close relationship with the level of IL-6. MMPs have diverse effects in promoting angiogenesis, remodeling of extracellular matrix and endothelial cell proliferation.

keywords:

interleukin-6, metalloproteinases, hemi-hepatectomy, rats, liver regeneration

Quick links
© 2022 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.