Interactions between neutrophils and chondrocytes seeded on scaffolds in an environment supplemented with synovial fluid exposed to the influence of variable atmospheric pressure

Implantation of an implant into the body can cause several side effects resulting in clinical problems. Activated neutrophils are responsible for most of them. In most published studies on the relationship between scaffolds and neutrophils in vitro, it is reported that the death of neutrophils is not a typical apoptosis or necrosis and is associated with excessive production of oxygen free radicals. In this study, we examined the relationship between scaffold and neutrophils under conditions similar to those prevailing in the pond. Scaffolds were settled by chondrocytes, and were incubated in different media: typical medium (DMEM/F12), medium supplemented with 50% FBS, or medium supplemented with physiological concentrations of synovial fluid (SF), collected from patients with rheumatoid arthritis. Cell cultures were exposed to the influence of variable atmospheric pressure. We demonstrated that death of neutrophils in the above experimental conditions is typically necrotic. These studies suggest that only experimental conditions maximally similar to physiological circumstances can provide reliable information about the course of events occurring in vivo