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1/2010
vol. 6
 
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Left ventricular diastolic abnormalities and the impact of hepatitis C virus infection in multitransfused Egyptian children

Maha M. El-Waseef
,
Safaa Taha
,
Hala Elgindi

Arch Med Sci 2010; 6, 1: 96-99
Online publish date: 2010/03/09
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Introduction

Multitransfused infants and children are those who have received two or more units of blood (Pineda et al., 1987). Blood transfusion was the principal transmission route of HCV infection in children. In a random healthy Egyptian sample of children the prevalence of HCV antibody seropositivity was relatively high (up to 12%) and significantly higher (up to 44%) in patients with thalassaemia and multitransfusion (El-Nanawy et al., 1995).
Waldes-Cruz et al. (1982) demonstrated abnormalities of left ventricular systolic and diastolic functions of asymptomatic children with b-thalassaemia. Rinz et al. (1999) and Francisco et al. (2006) found HCV replication in myocardial tissue of patients with myocarditis. Their study suggested that HCV infection may be involved in the development of an unusual form of idiopathic cardiomyopathy. Matsumori's (2001) study of idiopathic cardiomyopathy found that hepatitis C virus antibody was detected in 10.6% of patients with hypertrophic cardiomyopathy and in 6.3% with dilated ones. The present study aimed to evaluate left ventricular cardiac systolic and diastolic functions by echocardiography in multitransfused children and the possible risk of hepatitis C virus infection as an additional factor impairing cardiac functions in these patients.

Material and methods

This study was performed in the National Heart Institute in Cairo in 2003. Eighty Egyptian children from the health insurance clinic with history of multitransfusion were included. In 25 of them (patients), HCV antibody was detected. A sample of 20 normal children of the same age and sex were studied as a control group. Ninety percent of the multitransfused patients were diagnosed as having thalassaemia major and 10% with other blood disorders. These patients were subjected to a full history and thorough physical examination including weight, height, blood pressure and heart rate, duration of the disease and serum ferritin in the previous year.
All patients were screened for hepatitis C virus by HCV detection test ELISA (enzyme linked immunoassay) Atlas Link Biotech Co, Catalogue No. 921.
A complete cross-sectional echocardiographic imaging and Doppler examination of all the cardiac chambers was done to exclude left ventricular dysfunction by echocardiography Doppler examination. All echocardiographic measurements were done by the same operator and reported as the average of at least three cardiac cycles according to the criteria of the American Society of Echocardiography (Shan et al., 1978). Analysis of left ventricular systolic function detected from parasternal short axis and measuring the diastolic and end systolic diameters was performed. Posterior wall and septal wall thickness and fractional shortening (FS) were calculated. Analysis of left ventricular diastolic function was evaluated by pulsed Doppler sampling of the mitral inflow. The peak E (E) and A (A) wave velocities, the E/A ratio, deceleration time, and isovolumetric relaxation time (IVRT) were obtained.
Statistical analysis
Data are represented as mean (SD). Significant values were considered at a probability of p < 0.05. Linear regression analysis was tested between haematological and echocardiographic findings.

Results

Demographic data of the patients and healthy children are summarized in Table I. Weight, height and body surface area were significantly lower in the multitransfused group; furthermore, mean blood pressure was low, while heart rate showed a slight increase.
Table II comparing the multitransfused children who are HCV seropositive and HCV seronegative groups showed no significant difference between any of the parameters.
The haematological profile of the patients is shown in Table III. The mean pre-transfusion haemoglobin concentration was 7.5 ±0.3 g/dl and the average serum ferritin level was 1350 ±989 ng/ml. The majority of patients who had iron chelation showed good compliance with iron chelation treatment. Seventy percent of patients underwent splenectomy.
Among all haematological data considered in Table III, only serum ferritin concentration showed a weak negative correlation with left ventricular fractional shortening.

Discussion

This study shows interesting results concerning the abnormalities in systolic and diastolic function of the left ventricle in multitransfused Egyptian children without clinical cardiopulmonary involvement and a mean pre-transfusion haemo-globin concentration around 7.5 ±0.3 g/dl.
There is an increase in left ventricular volumes with decreased systolic and diastolic blood pressure. The increased volume load is a reflection of the Frank-Starling mechanism and an increase of heart rate. These findings are in agreement with those reported by others and are related to increased cardiac output caused by chronic anaemia (Kermastinos et al., 1993; Duke and Abelmann 1969).
The study shows a decrease in left ventricular systolic performance which is probably secondary to iron toxicity (Waldes et al., 1982; Christina et al., 2006). The results indicate significant left ventricular systolic dysfunction in HCV seropositive patients and this shows the implication of hepatitis C virus as an additional factor in cardiomyopathy. Matsumori (2001) suggested that hepatitis C virus is frequently found in patients with dilated cardiomyopathy (Bahl et al., 1998).
Reports concerning left ventricular diastolic function in patients with b-thalassaemia are somewhat conflicting (Spirito et al., 1990). In the study of Yaprak et al., 1998, 54% of patients having b-thalassaemia major had restrictive left ventricular diastolic abnormalities, and this correlates with our study, as a total of 56% of patients had diastolic dysfunction.
In the early phase of thalassaemia major before the appearance of systolic abnormalities with no symptoms of congestive heart failure, diastolic abnormalities were detected (Yapark et al., 1998; Iarussi et al., 2003; and Christina et al., 2006). Systolic dysfunction could be due to hepatitis C virus infection as reported by Matsumori and Sasayama (2000). In our study we found a weak but significant correlation between left ventricular fractional shortening and serum ferritin concentration, and this agreed with Bosi et al., 2003. In 1994 Oliveri et al. found that the cardiovascular prognosis in thalassaemic patients was excellent if serum ferritin concentration was maintained below 2500 ng/ml; this value was considered a safe concentration. However, Bosi et al. 2003 suggest that a serum ferritin value of less than 1000 ng/ml should be considered.
In conclusion, our study suggested that dilated cardiomyopathy in multitransfused children, especially thalassaemic patients, had several factors which could be involved in the pathogenesis. The first factor is the significant volume overload imposed by chronic anaemia. The second factor is hepatitis C virus infection, which could be an important causal agent. The third factor is iron toxicity, although the mechanism involved in this is not universally agreed. Cardiac examination and follow-up by Doppler echocardiography is an essential and important non-invasive safe tool for follow-up of these children. Chelation treatments and good compliance to maintain ferritin levels less than 1000 ng/ml give a good cardiovascular prognosis.

References


1. Bahl VK, Chandra S, Boro AK. Pulsed Doppler echocardiograhic study of left ventricular diastolic function in patients with idiopathic dilated cardiomyopathy. J Assoc Phys India 1998; 46: 257-60.
2. Bosi G, Crepaz R, Gamberini MR. Left ventricular remodeling, and systolic abnormalities in children with beta-thalassmia major: a Doppler echocardiographic assessment and correlation with haematological data. Heart 2003; 89: 762-6.
3. Christina C, Michael G, Christina P. Diastolic function in young patients with beta thalassemia major: an echocardiographic study. Echocardiogr J CV Ultrasound Allied Tech 2006; 23: 38-44.
4. Duke M, Abelmann WH. The haemodynamic response to chronic anemia. Circulation 1969; 34: 503-15.
5. El-Nanawy AA, El-Azzouni O, Soliman AT, et al. Prevalence of hepatitis C antibody seropositivity in healthy Egyptian children and four high risk groups. J Trop Ped 1995; 41: 341-3.
6. Dos Reis FJ, de Sousa TA, Oliveira MS, et al. Is Hepatitis C virus a cause of idiopathic dilated cardiomyopathy? A systematic review of literature. Braz J Infect Dis 2006; 10: 199-202.
7. Iarussi D, Di Salvo G, Pergola V, Pulsed Doppler tissue imaging and myocardial function in thalassemia major. Heart Vessels 2003; 18: 1-6.
8. Kremastinos DT, Rentoukas E, Mavrogeni S, Kyriakides ZS, Politis C, Toutouzas P. Left ventricular inflow pattern in beta-thalassemia major: a Doppler echocardiographic study. Eur Heart J 1993; 14: 351-7.
9. Kremastinos DT, Tsiapras DP, Tsetsos GA, Rentoukas EI, Vretou HP, Toutouzas PK. Left ventricular diastolic Doppler characteristics in beta-thalassemia major. Circulation 1993; 88: 1127-35.
10. Leon MB, Borer JS, Bacharach SL, et al. Detection of early cardiac dysfunction in patients with severe beta-thalassemia and chronic iron overload. N Engl J Med 1979; 301: 1143-8.
11. Matsumori A. Hepatitis C virus and cardiomyopathy. Herz 2001; 23: 249-54.
12. Matsumori A, Sasayama S. Idiopathic cardiomyopathy – pathogenesis. Nippon Rinsho 2000; 58: 12-7.
13. Oliveri NF, Nathan DG, MacMillan JH, et al. Survival in medically treated patients with homozygous beta-thalassemia. N Engl J Med 1994; 331: 574-8.
14. Pineda AA, Chase GJ, Taswelj HE. In: Thalassemia today. Siechia G, Zanella A (eds). Centro transfusionale Mila no 1987; 57.
15. Ruiz-Moreno M, Leal-Orozo A, Millan A. Hepatitis C virus infection in children. J Hep1999; 31 (Suppl. 1): 124-9.
16. Shan DJ, DeMaria A, Kisslo J, Weyman A. Recom-mendations regarding quantitation in M-mode echocardiography: result of a survey of echocardiographic measurements. Circulation 1978; 58: 1072-80.
17. Spirito P, Lupi G, Melevendi C, Vecchio C. Restrictive diastolic abnormalities identified by Doppler echocardiographic study in patients with thalassemia major. Circulation 1990; 82: 88-94.
18. Waldes Cruz LM, Reinecke C, Rutkowski M, et al. Preclinical abnormal segmental cardiac manifestations of Thalassemia major in children on transfusion-chelation therapy: echocardiographic alterations of left ventricular posterior wall contraction and relaxation pattern. Am Heart J 1982; 103: 505-11.
19. Yaprak I, Akisit S, Ozturk C, Nakiler AR, Dorak C. Left ventricular diastolic abnormalities in children with beta-thalassemia major: a Doppler echocardiographic study. Turk J Pediatr 1998; 40: 201-9.
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