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Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii
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vol. 37
Original paper

Long-term effects of allergen sublingual immunotherapy

Andrzej Bozek
Aleksandra Foks
Karolina Trzaska
Giorgio Walter Canonica

Clinical Department of Internal Diseases, Dermatology and Allergology in Zabrze, Medical University of Silesia in Katowice, Poland
Humanitas University, Milano, Italy
Adv Dermatol Allergol 2020; XXXVII (6): 943–947
Online publish date: 2019/05/27
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Allergen-specific immunotherapy (AIT) is a common therapy of many IgE-mediated diseases: allergic rhinoconjunctivitis, some cases of bronchial asthma and hypersensitivities to bee and wasp venom [1–3]. Allergen-specific immunotherapy has also been attempted in other allergic diseases. Allergen-specific immunotherapy has been shown to be effective and safe in many trials [4, 5]. Nevertheless, AIT is an important tool in modern allergology, although many doubts are not resolved. For instance, can AIT produce sufficient long-term allergen immunotolerance that is still persistent after this therapy is discontinued? There are especially few studies on sublingual allergen immunotherapy (SLIT) [6–8].
Currently, there is much evidence that SLIT is safe and effective, similar to injection allergen immunotherapy (SCIT) [1]. However, SCIT is frequently perceived as a therapy that gives a more long-term effect.


The objective of this study was to evaluate the clinical long-term effect of SLIT on common inhalant allergens in patients with allergic rhinitis when immunotherapy was discontinued.

Material and methods

Study design

This trial was a prospective follow-up observation of patients with allergic rhinitis who completed SLIT therapy in randomized, double-blind, placebo-controlled trials [9, 10]. During further observation, patients receiving SLIT were compared with those who were given placebo. There were two parallel observations: A – patients after SLIT or placebo for grass pollen; B – patients after SLIT for house dust mites (HDM).
Observation A: 47 patients with perennial allergic rhinitis (65.8 ±4.9 years old) underwent 3 years of SLIT for house dust mites and were compared to the placebo group [9]. Staloral 300 IR Dermatophagoides pteronyssinus and D. farinae 50/50 were used (Stallergenes Greer, London, UK). The combined symptom medication score (SMS), average adjusted symptom score (AAdSS) and IgG4 were measured at the start and end of immunotherapy.
Observation B: 68 patients with intermittent allergic rhinitis (63.2 ±3.1 years old) underwent pre- and co-seasonal SLIT of 3 years for grass pollen and were compared to the placebo group [10]. Staloral 300 IR grass extract was used (Stallergenes Greer, London, UK). Similar parameters were measured as mentioned above.
In this study, the prospective observations started at the moment when SLIT (or the placebo) was finished in each group.


The characteristics of the patients after SLIT, just before observation, are presented in Table 1 for house dust mites SLIT and in Table 2 for grass pollen SLIT.


The patients were asked to keep a diary during the entire 5-year observation period of 2013–2018. Every year (May–June for observation B and October-November for observation A), the following were performed:
1. Analysis of symptoms score and drug consumption based on the diary
2. Measurement of specific IgE and IgG4 levels for D. pteronyssinus or Phleum pratense (depending on the type of SLIT)
3. Assessment of quality of life using the Rhinoconjunctivitis Quality of Life Questionnaire (RQLQ).

Symptoms and medication score (SMS)

Patients were monitored for their clinical allergy symptoms and medication use from 2013 to 2018.
Patients recorded their nasal and ocular symptoms and medication use every day during the observation period (3 years during the trials and 5 years post-observation). Four nasal symptoms (sneezing, rhinorrhea, pruritus and congestion) and two ocular symptoms (pruritus and tears) were monitored. Each day, the patient rated the severity of each individual symptom over the past 24 h on a four-point scale: 0 – no symptoms; 1 – mild symptoms; 2 – moderate symptoms; and 3 – severe symptoms [11].
The rescue medication score was based on the World Allergy Organization (WAO) recommendations: 1 point for antihistamines, 2 points for nasal corticosteroids and 3 points for oral corticosteroids [11].
Symptoms and medication scores were presented as the combined symptom medication score (SMS). Additionally, the total combined rhinitis score (TCRS) was also measured. This score focuses on nasal domain symptoms and the medication used for allergic rhinitis [12].
All symptoms and medication scores obtained during SLIT (before the start of follow-up) were recalculated post hoc according to the method described above and used for the analysis.

Symptomatic treatment

Patients were allowed to use the following drugs: antihistamine (5 mg levocetirizine tablets), intranasal corticosteroid (mometasone), topical ocular antihistamine drops (ebastine) and methylprednisolone (4 mg tablets). Other anti-allergic drugs were not permitted during the observation period.

Allergen-specific IgE and IgG4

At the start, at the end and in every year of observation, serum-specific IgE and IgG4 levels for D. pteronyssinus and to Phleum pratense were determined using ImmunoCAP (Thermo Fisher Scientific, Uppsala, Sweden) following the manufacturer’s instructions. The results were considered positive when the sIgE concentration was greater than 0.35 IU/ml (according to the manufacturer’s instructions).
Additionally, the results of IgE and IgG4 and the appropriate allergens that were obtained during SLIT trials were also used in the final analysis.

Statistical analysis

The primary endpoint was the change of mean SMS and the TCRS during the 5 years of observation and compared with placebo.
The second point was assessment of the quality of life during the 5 years after SLIT. The persistence of the immune response to SLIT based on changes in the concentration of allergen-specific IgE and IgG4 was monitored.
Descriptive analyses were performed using Student’s t-test or the Wilcoxon test. The c2 test was used to analyse changes in quality of life. Analyses were performed using Statistica 8.12 (SoftPol, Cracow, Poland). P < 0.05 was considered statistically significant.


Five years after SLIT was discontinued, a significant clinical effect based on SMS and TCRS was still observed compared with the baseline (before SLIT) and the placebo groups. During the subsequent 5 years of follow-up observation, the SMS in both SLIT groups analysed (observations A and B) was significantly lower than that in the placebo group.
The results are presented in Tables 3 and 4. The trends in TCRS are shown in Figure 1.

Immunological parameters

Serum-specific IgE against D. pteronyssinus decreased during the SLIT trial and remained at the same levels 5 years after immunotherapy was discontinued compared to the placebo group. A similar trend was observed for patients who underwent SLIT for grass pollen (Table 5).
Serum-specific IgG4 against D. pteronyssinus increased during the SLIT trial in the study group. During the 5 years of observation after SLIT, there were no significant changes in specific IgG4 levels for the analysed allergens compared to results just after SLIT. However, the level of IgG4 for Phleum pratense decreased but not significantly in the fourth and fifth years of observation (Table 6).

Quality of life

Quality of life based on the RQLQ was significantly improved in patients who received SLIT and remained at a good level after 5 years of observation: 1) for SLIT for HDM (RQLQ mean score at baseline; just after SLIT; after 5 years’ follow-up): 1.48 (95% CI: 1.33–1.79); 1.01 (95% CI: 0.82–1.15); 1.13 (95% CI: 0.78–1.24), respectively, 2) for SLIT for grass pollen (RQLQ mean score at baseline; just after SLIT; after 5 years’ follow-up): 1.59 (95% CI: 1.26–1.89); 0.97 (95% CI: 0.62–1.43); 1.1 (95% CI: 0.88–1.31), respectively.


The long-term clinical and immunological effects after the end of AIT are still studied with great interest. There are still enough data, especially for SLIT, to analyse this issue [6, 7]. The question about it is necessary because of the expectations of doctors and patients after AIT in the elderly patients.
In this study, the good clinical effect of SLIT with the use of different allergens and protocol of administration was confirmed. The significant decrease in the combined symptom medication score, which was obtained after both types of SLIT, stayed for a long time on a similar, satisfactory level. This was especially visible compared with the placebo. Additional evidence of this is a good quality of life, which was still maintained 5 years after the end of AIT.
These results were in accordance with other trials with SLIT for grass pollen [7] and for house dust mites [6, 13]. However, these cited trials are based on only 2 years of observation after SLIT was discontinued.
There were also significant improvements in TCRS based on the analysis of the rhinitis domain. Such results are crucial for patients because nasal problems significantly reduce quality of life [14].
Moreover, the trends in immunological parameters were also promising even after 5 years of observation: a mild decrease in allergen-specific IgE and an increase in IgG4. However, there were some patients, especially in the HDM group, in whom the allergen-specific IgE concentration did not decrease significantly during or after SLIT.
These results were similar to other studies, but such long observations after SLIT have not yet been carried out. It is worth emphasizing that the examined people were at a mature age, which is a particularly important additional benefit of this study.
The main limitation of the study is the relatively small groups of patients. This was the reason why authors decided to show two different studies with similar results.
We also did not take into account the effect of individual pollen season or mite exposure in our results. However, the trends of the results observed in the following years between the study groups and the placebo group were always different and stable during the entire observation period.


The positive effect obtained after SLIT for grass pollen or house dust mites was sustained for a long period of time after AIT. Additional studies are still needed.

Conflict of interest

The authors declare no conflict of interest.
Copyright: © 2019 Termedia Sp. z o. o. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (http://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
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