eISSN: 1896-9151
ISSN: 1734-1922
Archives of Medical Science
Current issue Archive Special issues Subscription
SCImago Journal & Country Rank

 
2/2006
vol. 2
 
Share:
Share:
more
 
 
abstract:

Original paper
Fibroblast growth factors promote pancreatic cell proliferation in normal and STZ – treated hamsters

Maya Inchovska
,
Vesselina Ogneva
,
Yordanka Martinova

Arch Med Sci 2006; 2, 2: 90-93
Online publish date: 2006/06/22
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 

Introduction:
In various organs cell proliferation and differentiation are controlled by growth factors. The knowledge concerning the implication of FGF family members in pancreatic development is still incomplete. The aim of the present study was to examine the structure characteristics, functional and proliferative activity of normal and diabetic hamster pancreatic cells following treatment with FGF 1, 2 and 7 in vitro.
Material and methods:
We have studied three groups of healthy hamsters – 5 hamsters at age pnd 1, 10 hamsters at age pnd 5 and 7 hamsters at age pnd 10. We induced experimental diabetes in female hamsters during the first 24 hours after mating of female and male hamsters by intraperitoneal streptozotocin (STZ) administration at a dose of 65 mg/kg. For the study we used only these animals from the litter that had a blood glucose level over 11 mmol/l. Three groups of diabetic hamsters were investigated – 20 hamsters at age pnd 1, 20 hamsters at age pnd 5 and 20 hamsters at age pnd 10. The following methods were used: H.E. staining, P/A.F. staining and histoautoradiography.
Results:
Light microscopic studies showed active synthetic processes in pancreatic cells under the influence of investigated FGFs. In our experimental model of diabetes the labelling index of pancreatic cells was higher than in the corresponding groups of healthy hamsters. The most important finding in our study was that FGF2 at a concentration of 10 ng/l has shown the most prominent effect in normal hamsters at age pnd 10 and in the streptozotocin-treated hamsters at age pnd 5.
Conclusions:
We conclude that FGF 2 stimulates the initial process of cell aggregation and cluster formation. Our study provides evidence for a positive regulatory effect of FGF1, FGF 2 and FGF 7 on the expansion of the pancreatic cell mass in normal and streptozotocin-treated hamsters.
keywords:

fibroblast growth factors 1, 2 and 7, cell proliferation, pancreas, diabetes

Quick links
© 2021 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe