eISSN: 1896-9151
ISSN: 1734-1922
Archives of Medical Science
Current issue Archive Special issues Subscription
SCImago Journal & Country Rank

 
2/2005
vol. 1
 
Share:
Share:
more
 
 
abstract:

Original paper
The Abbott LCx-MTB assay is more sensitive than culture for detection of Mycobacterium tuberculosis in respiratory specimens

Wojciech Krajewski
,
Marek Kasielski
,
Maciej Król
,
Piotr Białasiewicz
,
Agnieszka Makówka
,
Dariusz Nowak

Arch Med Sci 2005; 1, 2: 80-88
Online publish date: 2005/09/21
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Background: In countries with a high incidence of tuberculosis quick, reliable and cost effective testing for this disease is mandatory. With the introduction of new methods based on detection of amplificated DNA fragment (Abbott LCx Mycobacterium tuberculosis assay) re-evaluation of the diagnostic panel (smear, culture and BACTEC versus LCx) is needed. We conducted a prospective study comparing clinical usefulness of these tests in detecting Mycobacterium tuberculosis in respiratory specimens from four clinical settings mandating transfer of respiratory specimens.
Material and methods: Respiratory specimens (320 sputum specimens and 54 bronchoalveolar lavage fluid specimens) collected from 374 patients under investigation for tuberculosis at the four clinical centers were divided into 2 portions. One portion was used for standard staining and cultures (auramine-fluorochrome and Ziehl-Neelsen staining, culture on Loevenstein-Jensen medium and in BACTEC Middlebrook 12B vials) and the second one was investigated for presence of Mycobacterium tuberculosis with LCx -MTB assay.
Results: In patients with a final diagnosis of tuberculosis we found LCx test more sensitive than culture and bacterioscopy (93.3% versus 81.3%; p<0.01 and 41.0%; p<0.001, respectively); while there was no difference in specificity and negative predictive value between LCx and culture.
Conclusions: LCx test is an effective diagnostic tool in the setting of the central laboratory to cooperate with different, distant, clinical centers for detection of Mycobacterium tuberculosis in respiratory specimens from subjects belonging to a population with a high incidence of tuberculosis.
keywords:

tuberculosis; mycobacterium tuberculosis complex; direct amplification test; culture

Quick links
© 2021 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe