Abstract
4/2016
vol. 54
Original paper
Protective role of dexmedetomidine in unmethylated CpG-induced inflammation responses in BV2 microglia cells
- Department of Anesthesiology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210000, China
- Department of Anesthesiology, The First People’s Hospital of Changzhou, Changzhou, Jiangsu, 213000, China
Folia Neuropathol 2016; 54 (4): 382-391
Online publish date: 2016/12/27
Unmethylated CpG DNA, as a stimulatory molecule, has potent pro-inflammatory effects in the central nervous system (CNS). Dexmedetomidine (DEX) has been confirmed to exert anti-inflammatory effects in CNS. Our study was aimed to explore the effects of DEX on tumor necrosis factor-α (TNF-α) expression in unmethylated CpG DNA-challenged microglia. In vivo, after 3 d intracisternal injection of ODN1668, we evaluated the severity of meningitis with or without DEX via pathobiology method and detected the expression of TNF-α from molecular and protein levels. In vitro, we explored whether the ODN1668 could activate microglia to express TNF-α and the inhibition mechanism of DEX. Our results demonstrated that DEX could alleviate the severity of ODN1668-induced meningitis. And while BV2 microglia was stimulated by ODN1668 for different time, TNF-α was increased in mRNA and protein levels but the effect was attenuated by DEX via decreasing phosphorylated AKT and ERK
Keywords
DEX, unmethylated CpG DNA, microglia
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