eISSN: 2299-0046
ISSN: 1642-395X
Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii
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SCImago Journal & Country Rank
5/2018
vol. 35
 
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abstract:
Original paper

Sputum interleukin-25 correlates with asthma severity: a preliminary study

Magdalena Paplińska-Goryca, Elżbieta M. Grabczak, Marta Dąbrowska, Joanna Hermanowicz-Salamon, Małgorzata Proboszcz, Patrycja Nejman-Gryz, Marta Maskey-Warzęchowska, Rafał Krenke

Adv Dermatol Allergol 2018; XXXV (5): 462-469
Online publish date: 2018/07/19
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Introduction
Interleukin 25 is an epithelial-derived cytokine associated with allergic Th2 inflammation. However, little is known about the role of IL-25 in different asthma phenotypes and its relationship with disease severity.

Aim
To evaluate and compare the mRNA and protein expression of IL-25 in patients with mild-to moderate/severe asthma and cough variant asthma (CVA).

Material and Methods
Thirty-eight patients with stable asthma (11 patients with mild-to-moderate asthma, 14 patients with severe asthma and 13 patients with CVA) and 14 control subjects were enrolled. IL-25 protein concentration was measured in induced sputum (IS) supernatants by ELISA and IL-25 mRNA expression was evaluated in IS cells by real time PCR.

Results
No differences in IS IL-25 mRNA and IL-25 concentration between controls and the whole asthma group were found. In the detailed analysis, a lower IL-25 mRNA expression in sputum cells was observed in severe asthma compared to CVA and controls. IL-25 protein concentration in sputum supernatants was elevated in patients with severe asthma compared to controls, CVA and mild-to-moderate asthma. A sputum IL-25 level was increased in atopic vs. non-atopic asthma patients. The elevated IL-25 mRNA expression and protein concentration was associated with a lower eosinophil and higher neutrophil percentage in asthmatic airways.

Conclusions
Our results suggest that IL-25 is particularly associated with severe asthma. The relationship between IL-25 and neutrophilic airway inflammation suggests the pleiotropic role of IL-25 in the immune response in this disease.

keywords:

interleukin 25, asthma, neutrophils, eosinophils, induced sputum

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