eISSN: 2084-9869
ISSN: 1233-9687
Polish Journal of Pathology
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vol. 69
Original paper

Steatosis influences hepatocytes proliferative potential in chronic hepatitis C patients

Andrzej Gabriel, Michał Kukla, Brygida Adamek, Kamil Tabor, Grzegorz Banasik, Maciej Horyniecki, Waldemar Halota

Pol J Pat hol 2018; 69 (4): 388-394
Online publish date: 2019/01/31
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The study evaluates the influence of steatosis on hepatocytes proliferative potential, reflected by proliferating cell nuclear antigen (PCNA) expression in chronic hepatitis C (CHC) patients both in steatotic and non-steatotic areas of lobules. The liver histology was evaluated according to Kleiner’s score. Nonalcoholic steatohepatitis (NASH) was also defined as the presence of lobular inflammation, hepatocyte ballooning and steatosis. Expression of PCNA was significantly in patients with definite NASH compared to those with simple steatosis, but not to those with borderline NASH. Advanced steatosis negatively influenced PCNA expression. NASH not only affects PCNA expression in staetotic, but also in non-steatotic lobule areas.

Expression of PCNA could be an independent indicator of changes in hepatocyte metabolism in CHC patients. High NAS values and low PCNA expression may be a negative prognostic factor in predicting the further course of the disease.

chronic hepatitis C, PCNA, nonalcoholic fatty liver disease, steatosis, hepatocyte ballooning

Kukla M, Piotrowski D, Waluga M et al. Insulin resistance and its consequences in chronic hepatitis C. Clin Exp Hepatol 2015; 1: 17-29.
Sanyal AJ. Review article: non-alcoholic fatty liver disease and hepatitis C-risk factors and clinical implications. Aliment Pharmacol Ther 2005; 22 Suppl 2: 48-51.
Masarone M, La Mura V, Bruno S, et al. Steatohepatitis is associated with diabetes and fibrosis in genotype 1b HCV-related chronic liver disease. J Viral Hepat 2007; 10: 714-720.
Hui JM, Sud A, Farrell GC, et al. Insulin resistance is associated with chronic hepatitis C infection and fibrosis progression. Gastroenterology 2003; 125: 1695-1704.
Kukla M, Gabriel A, Sabat D, et al. Association between liver steatosis and angiogenesis in chronic hepatitis C. Pol J Pathol 2010; 61: 154-160.
Muzzi A, Leandro G, Rubbia-Brandt L, et al. Insulin resistance is associated with liver fibrosis in non-diabetic chronic hepatitis C
patients. J Hepatol 2005; 42: 41-46.
Negro F. Mechanisms and significance of liver steatosis in hepatitis C virus infection. World J Gastroenterol 2006; 12: 6756-6765.
Perlemuter G, Sabile A, Letteron P, et al. Hepatitis C virus core protein inhibits microsomal triglyceride transfer protein activity and very low density lipoprotein secretion: a model of viral-related steatosis. FASEB J 2002; 16: 185-194.
Kao CC, Yi G, Huang HC. The core of hepatitis C virus pathogenesis. Curr Opin Virol 2016; 17: 66-73.
De Biasio A, Blanco FJ. Proliferating cell nuclear antigen structure and interactions: too many partners for one dancer? Adv Protein Chem Struct Biol 2013; 91: 1-36.
Eldridge SR, Butterworth BE, Goldsworthy TL. Proliferating cell nuclear antigen a marker for hepatocellular proliferation in rodents. Environ Health Perspect 1993; 101: 211-218.
Ojanguren I, Ariza A, Llatjos M, et al. Histochemical detection of proliferating cell nuclear antigen. Hum Pathol 1993; 24: 905-908.
Terada T, Nakanuma Y. Cell proliferative activity in adenomatous hyperplasia of the liver and small hepatocellular carcinoma: an immunohistochemical study demonstrating proliferating nuclear cell antigen. Cancer 1992; 70: 591-598.
Scheuer PJ. The nomenclature of chronic hepatitis: time for a change. J Hepatol 1995; 22: 112.
Kleiner DE, Brunt EM, Van Natta M, et al. Design and validation of histological scoring system for non alcoholic fatty liver disease. Hepatology 2005; 41: 1313-1321.
Hamada M, Kihira T, Takase K, et al. Hepatocyte regeneration in chronic hepatitis C and interferon treatment: analysis of immunohistological identification of proliferating cell nuclear antigen (PCNA). J Gastroenterol 1995; 30: 372-378.
Nakamura T, Hayama M, Sakai T, et al. Proliferative activity of hepatocytes in chronic viral hepatitis as revealed by immunohistochemistry for proliferating cell nuclear antigen. Hum Pathol 1993; 24: 750-753.
Persico M, Perrotta S, Persico E, et al. Hepatitis C virus carriers with persistently normal ALT levels: biological peculiarities and update of the natural history of liver disease at 10 years.
J Viral Hept 2006; 13: 290-296.
Juríková M, Danihel Ľ, Polák Š, Varga I. Ki67, PCNA, and MCM proteins: Markers of proliferation in the diagnosis of breast cancer. Acta Histochem 2016; 118: 544-552.
Wang SC. PCNA: a silent housekeeper or a potential therapeutic target? Trends Pharmacol Sci 2014; 35: 178-186.
Aaltomaa S, Lipponen P, Syrjänen K. K. Proliferating cell nuclear antigen (PCNA) immunolabeling as a prognostic factor in axillary lymph node negative breast cancer. Anticancer Res 1993; 13: 533-538.
Hu WT, Li HC, Lee SK, et al. Both core and F proteins of hepatitis C virus could enhance cell proliferation in transgenic mice. Biochem Biophys Res Commun 2013; 435: 147-152.
Chang ML. Metabolic alterations and hepatitis C: From bench to bedside. World J Gastroenterol 2016; 22: 1461-1476.
Wu Y, Chen K, Liu X, et al. SREBP-1 interacts with c Myc to enhance somatic cell reprogramming. Stem Cells 2016; 34: 83-92.
Cheng Y, Dharancy S, Malapel M, et al. Hepatitis C virus infection down-regulates the expression of peroxisome proliferator-activated receptor alpha and carnitine palmitoyl acyl-CoA transferase 1A. World J Gastroenterol 2005; 11: 7591-7596.
Kukla M, Berdowska A, Gabriel A, et al. Association between hepatic angiogenesis and serum adipokine profile in non-obese chronic hepatitis C patients. Pol J Pathol 2011; 62: 218-228.
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