eISSN: 2084-9869
ISSN: 1233-9687
Polish Journal of Pathology
Current issue Archive Manuscripts accepted About the journal Supplements Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank
vol. 69
Original paper

The influence of fibronectin on proliferation and apoptosis of acute lymphoblastic leukaemia cells in vitro

Joanna Włodek, Anna Pituch-Noworolska

Pol J Pathol 2018; 69 (1): 62-66
Online publish date: 2018/05/07
View full text
Get citation
JabRef, Mendeley
Papers, Reference Manager, RefWorks, Zotero
The extracellular matrix (ECM) is a dynamic environment involved in the regulation of haematopoiesis. A crucial role of this structure is the promotion of proliferation, maturation, and differentiation of haematopoietic stem cells (HSC), and adhesion and migration of HSC in bone marrow.

In the present study the effect of ECM proteins (fibronectin, collagens, laminin, thrombospondin, and vitronectin) on proliferation and apoptosis of acute lymphoblastic leukaemia cells isolated from acute lymphoblastic leukaemia (ALL) patients (in vitro) was assessed. The leukaemia cells were obtained as interphase on Ficoll/Isopaque (Pancoll human, PAN-Biotech) density gradient and, after washing, counted in a chamber. Subsequently, cells were used for culture and apoptosis assay.

Presence of fibronectin, collagen type IV, and laminin was associated with inhibition of lymphoblastic leukaemia cell proliferation. Analysis of the culture of lymphoblastic leukaemia cells in the presence of ECM showed fibronectin as the most active protein.

acute lymphoblastic leukaemia, proliferation, apoptosis, fibronectin, collagen

Dworzak MN, Buldini B, Gaipa G. AIEOP-BFM consensus guidelines 2016 for flow cytometric immunophenotyping of pediatric acute lymphoblastic leukemia, Cytometry B Clin Cytom 2018; 94: 82-93.
Choi JS, Harley BA. Marrow-inspired matrix cues rapidly affect early fate decision of hematopoietic stem and progenitor cells. Sci Adv 2017; 3: 1-10.
Song K, Li L, Wang Y, et al. Hematopoietic stem cells: multiparameter regulation. Human Cell 2016; 29: 53-57.
Sangaletti S, Chiodoni C, Tripodo C, et al. Common extracellular matrix regulation of myeloid cell activity in the bone marrow and tumor microenvironments. Cancer Immunol Immunother 2017; 66: 1059-1067.
Favreau AJ, Vary CP, Brooks PC et al. Cryptic collagen IV promotes cell migration and adhesion in myeloid leukemia. Cancer Med 2014; 3: 265-272.
Chiarini F, Lonetti A, Evangelisti C et al. Advances in understanding the caute lymphoblastic leukemia bone marrow microenviroment. Biochem Biophys Acta 2016; 1863: 449-463.
Zollinger AJ, Smith ML. Fibronectin, the extracellular glue. Matrix Biol 2017; 60: 27-37.
Lazar-Karsten P, Dorn I, Meyer G. The influence of extracellular matrix proteins and mesenchymal stem cells on erythropoietic cell maturation. Vox Sang 2011; 101: 65-76.
Shin JW, Mooney DJ. Extracellular matrix stiffness causes systematic variations in proliferation and chemosensitivity in myeloid leukemias. Proc Natl Acad Sci U S A 2016; 113: 12126-12131.
Dutta A, Sen T, Chatterjee A. Culture of K562 human myeloid leukemia cells in presence of fibronectin expresses and secretes MMP-9 in serum-free culture medium. Int J Clin Exp Pathol 2010; 3: 288-302.
Quick links
© 2019 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe