<i>Experimental immunology</i><br>Anti-Toll-like receptor 2 antibody, T2.1,
reduces tumor necrosis factor α and
interleukin-6 release and inhibits nuclear
factor κB expression in bacterial lipoprotein
stimulated THP-1 cells

Min Huang; Jing Zhou; Liqing Bi; Qian Zhang; Suming Zhou

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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abstract:

Experimental immunology
Anti-Toll-like receptor 2 antibody, T2.1, reduces tumor necrosis factor α and interleukin-6 release and inhibits nuclear factor κB expression in bacterial lipoprotein stimulated THP-1 cells

Min Huang

,

Jing Zhou

,

Liqing Bi

,

Qian Zhang

,

Suming Zhou

(Centr Eur J Immunol 2012; 37 (1): 6-10)

Online publish date: 2012/03/29

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AMA

Huang M, Zhou J, Bi L, Zhang Q, Zhou S. Experimental immunologyAnti-Toll-like receptor 2 antibody, T2.1,
reduces tumor necrosis factor α and
interleukin-6 release and inhibits nuclear
factor κB expression in bacterial lipoprotein
stimulated THP-1 cells. Central European Journal of Immunology. 2012;37(1):6-10.

APA

Huang, M., Zhou, J., Bi, L., Zhang, Q.,  & Zhou, S. (2012). Experimental immunologyAnti-Toll-like receptor 2 antibody, T2.1,
reduces tumor necrosis factor α and
interleukin-6 release and inhibits nuclear
factor κB expression in bacterial lipoprotein
stimulated THP-1 cells. Central European Journal of Immunology, 37(1), 6-10.

Chicago

Huang, Min, Jing Zhou, Liqing Bi, Qian Zhang,  and Suming Zhou. 2012. "Experimental immunologyAnti-Toll-like receptor 2 antibody, T2.1,
reduces tumor necrosis factor α and
interleukin-6 release and inhibits nuclear
factor κB expression in bacterial lipoprotein
stimulated THP-1 cells". Central European Journal of Immunology 37 (1): 6-10.

Harvard

Huang, M., Zhou, J., Bi, L., Zhang, Q.,  and Zhou, S. (2012). Experimental immunologyAnti-Toll-like receptor 2 antibody, T2.1,
reduces tumor necrosis factor α and
interleukin-6 release and inhibits nuclear
factor κB expression in bacterial lipoprotein
stimulated THP-1 cells. Central European Journal of Immunology, 37(1), pp.6-10.

MLA

Huang, Min et al. "Experimental immunologyAnti-Toll-like receptor 2 antibody, T2.1,
reduces tumor necrosis factor α and
interleukin-6 release and inhibits nuclear
factor κB expression in bacterial lipoprotein
stimulated THP-1 cells." Central European Journal of Immunology, vol. 37, no. 1, 2012, pp. 6-10.

Vancouver

Huang M, Zhou J, Bi L, Zhang Q, Zhou S. Experimental immunologyAnti-Toll-like receptor 2 antibody, T2.1,
reduces tumor necrosis factor α and
interleukin-6 release and inhibits nuclear
factor κB expression in bacterial lipoprotein
stimulated THP-1 cells. Central European Journal of Immunology. 2012;37(1):6-10.

This study analyzed the effects of a blocking anti-Toll-like receptor 2 (TLR2) antibody (T2.1) on TLR2 signaling and inflammatory cytokine production by bacterial lipoprotein (BLP) stimulated THP-1 cells. Cells were treated sequentially with anti-TLR2 antibody and BLP (A + B) or in the reverse order (B + A), or directly stimulated with BLP (B). Blank and isotype control groups were included. Tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) concentrations were measured by ELISA. Expression of nuclear factor κB (NF-κB), TLR2 and myeloid differentiation factor 88 (MyD88) was analyzed by Western Blot. Significantly higher expression of TNF-α, IL-6 and NF-κB was detected in all groups compared with the blank control (P < 0.05), although expression was significantly lower in A + B and B + A compared with the isotype control and B (P > 0.05). However, no significant differences were detected in TLR2 and MyD88 expression among these groups (P > 0.05). These data demonstrated that anti-TLR2 antibody significantly reduced TNF-α, IL-6 and NF-κB expression by BLP-stimulated THP-1 cells, thus indicating the protective value of this antibody in vivo.

keywords:

Experimental immunologyAnti-Toll-like receptor 2 antibody, T2.1, reduces tumor necrosis factor α and interleukin-6 release and inhibits nuclear factor κB expression in bacterial lipoprotein stimulated THP-1 cells

Min Huang , Jing Zhou , Liqing Bi , Qian Zhang , Suming Zhou

anti-TLR2 antibody, BLP, NF-κB, TNF-α, IL-6

Experimental immunology
Anti-Toll-like receptor 2 antibody, T2.1, reduces tumor necrosis factor α and interleukin-6 release and inhibits nuclear factor κB expression in bacterial lipoprotein stimulated THP-1 cells

Min Huang

,

Jing Zhou

,

Liqing Bi

,

Qian Zhang

,

Suming Zhou