eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank

1/2012
vol. 37
 
Share:
Share:
more
 
 
abstract:

Experimental immunology
Anti-Toll-like receptor 2 antibody, T2.1, reduces tumor necrosis factor α and interleukin-6 release and inhibits nuclear factor κB expression in bacterial lipoprotein stimulated THP-1 cells

Min Huang
,
Jing Zhou
,
Liqing Bi
,
Qian Zhang
,
Suming Zhou

(Centr Eur J Immunol 2012; 37 (1): 6-10)
Online publish date: 2012/03/29
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
This study analyzed the effects of a blocking anti-Toll-like receptor 2 (TLR2) antibody (T2.1) on TLR2 signaling and inflammatory cytokine production by bacterial lipoprotein (BLP) stimulated THP-1 cells. Cells were treated sequentially with anti-TLR2 antibody and BLP (A + B) or in the reverse order (B + A), or directly stimulated with BLP (B). Blank and isotype control groups were included. Tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) concentrations were measured by ELISA. Expression of nuclear factor κB (NF-κB), TLR2 and myeloid differentiation factor 88 (MyD88) was analyzed by Western Blot. Significantly higher expression of TNF-α, IL-6 and NF-κB was detected in all groups compared with the blank control (P < 0.05), although expression was significantly lower in A + B and B + A compared with the isotype control and B (P > 0.05). However, no significant differences were detected in TLR2 and MyD88 expression among these groups (P > 0.05). These data demonstrated that anti-TLR2 antibody significantly reduced TNF-α, IL-6 and NF-κB expression by BLP-stimulated THP-1 cells, thus indicating the protective value of this antibody in vivo.
keywords:

anti-TLR2 antibody, BLP, NF-κB, TNF-α, IL-6

FEATURED PRODUCTS
Quick links
© 2019 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe