Abstract
Cobas 4800 HPV detection in cervical samples of Polish women
- Laboratory of Cancer Genetics, Department of Pathology, Polish Mother’s Memorial Hospital Research Institute, Lodz, Poland
- Department of Obstetrics and Gynaecology and Gynaecological Oncology, Regional Hospital in Kalisz, Poland
- The President Stanisław Wojciechowski Higher Vocational State School in Kalisz, Poland
- Department of Operative Gynaecology, Polish Mother’s Memorial Hospital Research Institute, Lodz, Poland
- Klinikum Bad Saarow, Germany
- Maria Skłodowska-Curie Institute – Oncology Center, Branch in Cracow, Poland
Introduction
Long-term infection with human papillomavirus (HPV) is the cause of cervical cancer and its precursor – cervical intraepithelial neoplasia (CIN). The presence of HPV infection can be presumed in more than 99% of cases of cervical cancer worldwide. The introduction of DNA testing for the presence of HPV has increased the effectiveness of screening programs for the detection of this cancer. This study aimed to analyze the prevalence of high risk HPV DNA (HR HPV) in females from Poland.
Material and methods
The study was performed on 280 cervical smear samples. In this work we used the Roche Cobas 4800 HPV test to detect the HR HPV in cervical smear samples.
Results
56 patients (20%) proved to be positive regarding HPV-16 DNA and 40 patients (14.28%) regarding HPV-18 DNA. In overall assessment, in 94 patients (33.57%) we detected oncogenic HPV subtypes, other than the two mentioned above. In 90 patients (32.14%) no high risk HPV was detected.
Conclusions
The Roche Cobas 4800 HPV test is a viable, effective, easy and quick tool in detecting high risk HPV DNA.
Keywords
HPV, cervical cancer, genotyping
Coverage in
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