Cobas 4800 HPV detection in cervical samples of Polish women

Beata Smolarz; Dariusz Samulak; Krzysztof Szyłło; Marianna Makowska; Hanna Romanowicz; Zbigniew Kojs; Magdalena M. Michalska

doi:10.5114/wo.2019.85880

eISSN: 1897-4309
ISSN: 1428-2526

Contemporary Oncology/Współczesna Onkologia

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2/2019
vol. 23

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abstract:

Original paper

Cobas 4800 HPV detection in cervical samples of Polish women

Beata Smolarz

¹

,

Dariusz Samulak

^{2, 3}

,

Krzysztof Szyłło

⁴

,

Marianna Makowska

⁵

,

Hanna Romanowicz

¹

,

Zbigniew Kojs

⁶

,

Magdalena M. Michalska

^{2, 3}

Laboratory of Cancer Genetics, Department of Pathology, Polish Mother’s Memorial Hospital Research Institute, Lodz, Poland

Department of Obstetrics and Gynaecology and Gynaecological Oncology, Regional Hospital in Kalisz, Poland

The President Stanisław Wojciechowski Higher Vocational State School in Kalisz, Poland

Department of Operative Gynaecology, Polish Mother’s Memorial Hospital Research Institute, Lodz, Poland

Klinikum Bad Saarow, Germany

Maria Skłodowska-Curie Institute – Oncology Center, Branch in Cracow, Poland

Contemp Oncol (Pozn) 2019; 23 (2): 92-95

DOI: https://doi.org/10.5114/wo.2019.85880

Online publish date: 2019/06/13

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Introduction
Long-term infection with human papillomavirus (HPV) is the cause of cervical cancer and its precursor – cervical intraepithelial neoplasia (CIN). The presence of HPV infection can be presumed in more than 99% of cases of cervical cancer worldwide. The introduction of DNA testing for the presence of HPV has increased the effectiveness of screening programs for the detection of this cancer. This study aimed to analyze the prevalence of high risk HPV DNA (HR HPV) in females from Poland.

Material and methods
The study was performed on 280 cervical smear samples. In this work we used the Roche Cobas 4800 HPV test to detect the HR HPV in cervical smear samples.

Results
56 patients (20%) proved to be positive regarding HPV-16 DNA and 40 patients (14.28%) regarding HPV-18 DNA. In overall assessment, in 94 patients (33.57%) we detected oncogenic HPV subtypes, other than the two mentioned above. In 90 patients (32.14%) no high risk HPV was detected.

Conclusions
The Roche Cobas 4800 HPV test is a viable, effective, easy and quick tool in detecting high risk HPV DNA.

keywords:

Cobas 4800 HPV detection in cervical samples of Polish women

Beata Smolarz 1 , Dariusz Samulak 2, 3 , Krzysztof Szyłło 4 , Marianna Makowska 5 , Hanna Romanowicz 1 , Zbigniew Kojs 6 , Magdalena M. Michalska 2, 3

HPV, cervical cancer, genotyping

Beata Smolarz

¹

,

Dariusz Samulak

^{2, 3}

,

Krzysztof Szyłło

⁴

,

Marianna Makowska

⁵

,

Hanna Romanowicz

¹

,

Zbigniew Kojs

⁶

,

Magdalena M. Michalska

^{2, 3}