eISSN: 2299-551X
ISSN: 0011-4553
Journal of Stomatology
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vol. 77
Original paper

Comparative evaluation of tensile bond strength in healthy dentin and caries-affected dentin, pre-treated with either grape seed extract or riboflavin: an in vitro study

Prasanna Bipin Dhatavkar
Shalini Devindernath Aggarwal
Rhea Santosh Digholkar
Neil Vincent Lewis
A.K. Anjali
Vini Mehta

  1. Department of Conservative Dentistry and Endodontics, Dr. D. Y. Patil Dental College & Hospital, Dr. D. Y. Patil Vidyapeeth, Pune, India
  2. Dental Research Cell, Dr. D. Y. Patil Dental College & Hospital, Dr. D. Y. Patil Vidyapeeth, Pune, India
J Stoma 2024; 77, 2: 87-92
Online publish date: 2024/05/29
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Advances in composite resins have changed the way restorative dentistry is now performed. The objective of restorative dentistry is to replace lost hard tissue with restorative material that has a good prognosis and longevity. In spite of the great improvements in the field of adhesive dentistry, hybridization of caries-affected dentin has been broadly avoided by clinicians and researchers. The loss of mineral content during a caries process is further compounded by action of matrix metalloproteinases (MMPs) on collagen of dentin. Recently, inhibition of MMPs has been the focus of research.

To compare the effect of MMP inhibitors on the bond strength of healthy dentin and caries-affected dentin. MMP inhibitors selected for this study were grape seed extract and riboflavin.

Material and methods:
Thirty-six extracted teeth were divided into two groups: healthy dentin and caries- affected dentin (CAD). Samples were grounded to expose dentinal surfaces. CAD samples were exposed to 10% citric acid for 5 minutes to simulate caries. The exposed dentinal surfaces of samples in each group were then treated, either with grape seed extract or riboflavin, followed by application of eighth-generation bond-ing agent and restoration with a composite resin, standardized to a specific height and diameter. This assem-bly was then subjected to testing in a universal testing machine to evaluate tensile bond strength (TBS) of adhesive layers.

The average TBS of healthy dentin treated with grape seed extract was found to be the highest (34.28 MPa) amongst the groups. The lowest TBS was found in the group with caries-affected dentin treated with ribofla-vin. There was a marked increase in TBS obtained in CAD after the application of MMP inhibitors.

There is a significant difference between the bond strengths of healthy and caries-affected dentins. GSE and riboflavin both enhanced the bonding performance of eighth-generation bonding agent in both healthy dentin and CAD samples.


dentin, caries-affected dentin, matrix metalloproteinases, bond strength, adhesive dentistry, grape seed ex-tract

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