Ectromelia virus persistence revisited: virus detection by <i>in situ</i> PCR after long-term infection of BALB/c mice

Felix N. Toka; Cespedes Irma Spohr; Ada Schollenberger; Małgorzata Krzyżanowska; Małgorzata Gieryńska; Miao Ru; Marek Niemiałtowski

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures

Editorial System

Submit your Manuscript

1-2/2005
vol. 30

Send email

Copy url:

abstract:

**Ectromelia virus persistence revisited: virus detection by in situ PCR after long-term infection of BALB/c mice**

Felix N. Toka

,

Cespedes Irma Spohr

,

Ada Schollenberger

,

Małgorzata Krzyżanowska

,

Małgorzata Gieryńska

,

Miao Ru

,

Marek Niemiałtowski

Centr Eur J Immunol 2005; 30 (1-2): 17-25

Online publish date: 2006/07/26

View full text Get citation

We measured the extent of ectromelia virus (ECTV) persistence in experimentally infected BALB/c [H-2^d] by means of in situ PCR and report that ECTV persists in the spleen for at least 12 weeks. Importantly, ECTV maintained a low profile replication in the spleen of mice allowing it to persist considerably longer following initial infection in the absence of clinical disease. Recovery of the virus by cell culture was possible up to 8 weeks post infection. Since ECTV is an Orthopoxvirus that infects genetically susceptible mouse strains, the implication of our findings is that careful screening for this natural mouse pathogen using sensitive assays such as in situ PCR should be periodically carried out in colonies of susceptible mice. On the other hand ECTV and BALB/c [H-2^d] form a suitable study model for generalized viral infection. As such in the light of the data described here the model would suit investigations on the immune status of persistently infected animals.

keywords:

Ectromelia virus persistence revisited: virus detection by in situ PCR after long-term infection of BALB/c mice

Felix N. Toka , Cespedes Irma Spohr , Ada Schollenberger , Małgorzata Krzyżanowska , Małgorzata Gieryńska , Miao Ru , Marek Niemiałtowski

mousepox virus, ectromelia, persistence, in situ polymerase chain reaction

**Ectromelia virus persistence revisited: virus detection by in situ PCR after long-term infection of BALB/c mice**

Felix N. Toka

,

Cespedes Irma Spohr

,

Ada Schollenberger

,

Małgorzata Krzyżanowska

,

Małgorzata Gieryńska

,

Miao Ru

,

Marek Niemiałtowski