eISSN: 2299-551X
ISSN: 0011-4553
Journal of Stomatology
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vol. 75
Original paper

Effects of chemo-therapeutic drugs used in head and neck cancers on sub-mandibular glands of rats

Zeliha Zuhal Yurdabakan
Ilknur Ozcan
Seyhun Solakoğlu

  1. Department of Oral and Dentomaxillofacial Radiology, Altinbas University, Faculty of Dentistry, Turkey
  2. Department of Oral and Dentomaxillofacial Radiology, Biruni University, Faculty of Dentistry, Istanbul, Turkey
  3. Department of Histology and Embryology, Istanbul University, Istanbul Faculty of Medicine, Turkey
J Stoma 2022; 75, 2: 61-71
Online publish date: 2022/06/23
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Chemo-therapeutic drugs can cause morphological damages to the salivary glands.

This study aimed to analyze and elucidate 5-fluorouracil (5-FU), cisplatin, methotrexate (MTX), and adriamycin (ADR)-induced morphological changes on submandibular glands (SMGs) of the rats.

Material and methods
Forty male Wistar albino rats were divided into five groups, with 8 rats per group. In the control group, 1 ml/kg 0.9% sterile saline solution; in the second group, 100 mg/kg dose of 5-FU; in the third group, 5 mg/kg dose of cisplatin; in the fourth group, 15 mg/kg dose of MTX; in the fifth group, 15 mg/kg dose of ADR were injected once a day for three consecutive days intra-peritoneally. In all groups, SMGs were excised, histopathological analyses were done using a light microscope, and total protein content was analyzed according to Lowry method. Data were examined using analysis of variance (ANOVA), Tukey HSD, Kruskal-Wallis, and Dunn’s tests. Results were evaluated at 95% confidence interval and significance level of p < 0.05.

There was a statistically significant decrease in gland weight and total protein content of the 5-FU, ADR, and cisplatin groups compared to that of the control (p < 0.001). However, the decrease in gland weight and total protein content of MTX group was not statistically significant (p > 0.05). Acinus areas of 5-FU, ADR, cisplatin, and MTX groups were lower than that of the control significantly (p < 0.001).

According to morphological parameters of our study, the most toxic effects were found to be caused by 5-FU, followed by ADR and cisplatin, while MTX presented the least toxic effects on the rats’ SMGs.


5-FU, cisplatin, methotrexate, adriamycin, submandibular gland

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