eISSN: 2299-0046
ISSN: 1642-395X
Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii
Current issue Archive Manuscripts accepted About the journal Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank
2/2022
vol. 39
 
Share:
Share:
more
 
 
abstract:
Original paper

Effects of tea polyphenols on UVA-induced melanogenesis via inhibition of α-MSH-MC1R signalling pathway

Jiaoquan Chen
1
,
Huaping Li
1
,
Bihua Liang
1
,
Huilan Zhu
1

1.
Department of Dermatology, Guangzhou Institute of Dermatology, Guangzhou, Guangdong, China
Adv Dermatol Allergol 2022; XXXIX (2): 327-335
Online publish date: 2022/05/09
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Introduction and aim
Ultraviolet (UV) irradiation is a major environmental factor affecting photoaging, which is characterized by skin wrinkle formation and hyperpigmentation. Although many factors are involved in the melanogenesis progress, UV is thought to play a major role in tanning. The pathway of a-melanocyte-stimulating hormone (a-MSH)-melanocortin receptor 1 (MC1R) is associated with UV-induced melanogenesis. Thus, a-MSH antagonists may have applications in the prevention of melanogenesis.
Aim: To investigate the effects of tea polyphenols (TPS) on pigmentation, and further explore the underlying mechanism.

Material and methods
Human keratinocyte cell line (HaCaT) cells and Human epidermal melanocytes (HEM) were exposed to UVA and treated with different concentrations of TPS or Nonapeptide-1 acetate salt (N-1A). Then, cell viability, melanin content, and tyrosinase activity of both kinds of cells were detected. Quantification of a-MSH in HaCaT cells and HEM cells determined by ELISA assays. Immunohistochemistry of HEM cells was employed to further investigate the expression of melanogenesis-related proteins.

Results
The different concentrations of TPS were found to decrease the melanin content, tyrosinase activity and melanogenesis-related proteins such as microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP)1, and TRP2. Besides, TPS inhibited a-MSH-MC1R signalling through directly suppressed a-MSH expression rather than the down-regulated expression level of MC1R.

Conclusions
Our findings indicate that TPS may be a potential whitening agent for use in cosmetics and the medical treatment of hyperpigmentation disorders.

keywords:

UV, melanogenesis, tea polyphenols, a-MSH-MC1R

Quick links
© 2022 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.