eISSN: 2299-551X
ISSN: 0011-4553
Journal of Stomatology
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3/2018
 
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abstract:
Original paper

In vitro efficacy of garlic extract against Candida albicans biofilms from children with early childhood caries

Vidya Carolyn Tjokrosetio, Sarworini Bagio Budiardjo, Ike Siti Indiarti, Eva Fauziah, Margaretha Suharsini, Heriandi Sutadi, Mochamad Fahlevi Rizal

J Stoma 2018; 71, 3: 263-267
Online publish date: 2018/12/23
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Introduction
Garlic (Allium sativum) is known to be a natural antifungal agent with the ability to penetrate cellular and organelle membranes, resulting in cell death of Candida albicans.

Objectives
The aim of the present study was to measure the efficacy of garlic extract against C. albicans biofilms frequently detected in early childhood caries (ECC).

Material and methods
Samples of dental biofilms were obtained from children with ECC, cultured in CHROMagar Candida medium, and confirmed using a polymerase chain reaction (PCR) technique. Furthermore, biofilms were established in microplates, incubated for 48 h, and exposed to 10%, 25%, 50%, and 100% garlic extract using 0.2% chlorhexidine (CHX) as a positive control. Cell viability was subsequently measured by the methyl- thiazolyl-tetrazolium (MTT) assay.

Results
There was a statistically significant decrease in the viability of C. albicans biofilms at all concentrations of garlic extract (p ≤ 0.000).

Conclusions
Garlic extract is efficacious in reducing the viability of C. albicans biofilms from children with ECC.

keywords:

Candida albicans, ECC, garlic extract, biofilm, MTT assay

references:
Xiao J, Moon Y, Li L, et al. Candida albicans carriage in children with severe early childhood caries (S-ECC ) and maternal relatedness. PLoS One 2016; 11: 1-16.
Koo H, Bowen WH. Candida albicans and Streptococcus mutans: a potential synergistic alliance to cause virulent tooth decay in children. Future Microbiol 2014; 9: 1295-1297.
Falsetta ML, Klein MI, Colonne PM, et al. Symbiotic relationship between Streptococcus mutans and Candida albicans synergizes virulence of plaque biofilms in vivo. Infect Immun 2014; 82: 1968-1981.
Costa-Orlandi CB, Sardi JCO, Pitangui NS, et al. Fungal biofilms and polymicrobial diseases. J Fungi 2017; 3: 1-24.
Li W, Shi QS, Dai HQ, et al. Antifungal activity, kinetics and molecular mechanism of action of garlic oil against Candida albicans. Sci Rep 2016; 6: 22805.
Houshmand B, Mahjour F, Dianat O. Antibacterial effect of different concentrations of garlic (Allium sativum) extract on dental plaque bacteria. Indian J Dent Res 2013; 24: 71-76.
Li G, Ma X, Deng L, et al. Fresh garlic extract enhances the antimicrobial activities of antibiotics on resistant strains in vitro. Jundi­shapur J Microbiol 2015; 8: e14814.
Council R. Policy on early childhood caries (ECC): classifications, consequences, and preventive strategies. Am Acad Pediatr Dent 2016; 39: 59-61.
Yang XQ, Zhang Q, Lu LY, et al. Genotypic distribution of Candida albicans in dental biofilm of Chinese children associated with severe early childhood caries. Arch Oral Biol 2012; 57: 1048-1053.
Hwang G, Liu Y, Kim D, et al. Candida albicans mannans mediate Streptococcus mutans exoenzyme GtfB binding to modulate cross-kingdom biofilm development in vivo. PLoS Pathog 2017; 13: e1006407.
Ramage G, Vandewalle K, Wickes BL, López-Ribot JL. Characteristics of biofilm formation by Candida albicans. Rev Iberoam Micol 2001; 18: 163-170.
Borlinghaus J, Albrecht F, Gruhlke MCH, et al. Allicin: chemistry and biological properties. Molecules 2014; 19: 12591-12618.
Low CF, Chong PP, Yong PVC, et al. Inhibition of hyphae formation and SIR2 expression in Candida albicans treated with fresh Allium sativum (garlic) extract. J Appl Microbiol 2008; 105: 2169-2177.
Fujisawa H, Suma K, Origuchi K, et al. Biological and chemical stability of garlic-derived allicin. J Agric Food Chem 2008; 56: 4229-4235.
Peters BM, Ward RM, Rane HS, et al. Efficacy of ethanol against Candida albicans and Staphylococcus aureus polymicrobial biofilms. Antimicrob Agents Chemother 2013; 57: 74-82.
Rambet LG, Waworuntu O, Gunawan PN. Uji konsentrasi hambat hinimum (KHM) perasan murni bawang putih (Allium sativum) terhadap pertumbuhan Candida albicans. J Ilm Farm 2017; 6: 16-23.
Onsare JG, Arora DS. Antibiofilm potential of flavonoids extracted from Moringa oleifera seed coat against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. J Appl Microbiol 2014; 118: 313-325.
Bakhshi M, Taheri J, Shabestari SB, Tanik A. Comparison of therapeutic effect of aqueous extract of garlic and nystatin mouthwash in denture stomatitis. Gerodontology 2012; 29: 680-685.
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