eISSN: 2299-551X
ISSN: 0011-4553
Journal of Stomatology
Current issue Archive Manuscripts accepted About the journal Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
vol. 77
Original paper

Investigation of cytotoxicity and antibacterial effect of boron-containing nano-hydroxyapatite

Numan Aydın
Serpil Karaoğlanoğlu
Aysun Kılıç Süloğlu
Neslihan İdil
Merve Demir
İbrahim Hakkı Karakaş

  1. Department of Restorative Dental Treatment, University of Health Sciences, Gulhane Faculty of Dentistry, Ankara, Turkey
  2. Department of Biology, Faculty of Science, Hacettepe University, Ankara, Turkey
  3. Engineering Faculty, Bayburt University, Bayburt, Turkey
J Stoma 2024; 77, 2: 93-99
Online publish date: 2024/05/29
View full text Get citation
PlumX metrics:
Different regenerative materials are used to protect and stimulate pulp tissue.

This in vitro study aimed to examine the cytotoxicity of different doses of boron-containing nano-hydroxyapatite (B-nHAp) and its antibacterial effect.

Material and methods:
B-nHAp was synthesized with microwave-assisted chemical precipitation method at different rates, such as 1%, 5%, and 10%. The extracts were prepared in separate tubes: 1%, 5%, 10% B-nHAp, and nHAp 50 mg/ml. Cytotoxicity test was performed on L929 mouse fibroblasts for 24 and 72 hours. Antibacterial analysis was performed with Streptococcus mutans bacteria using micro-dilution method. Cell viability values were evaluated with two-way analysis of variance (ANOVA) test (p < 0.05).

B-nHAp was successfully synthesized in XRD and SEM analyses. Nano-hydroxyapatite showed more cell viability than control group at 24 and 72 hours. All B-nHAp extracts demonstrated less cell viability at the end of 24 hrs. than control group. However, 1% B-nHAp cell viability decreased more in the B-nHAp group compared with the control group, while 5% and 10% B-nHAp showed high cell viability after 72 hours of incubation. B-nHAp demonstrated less antibacterial effect on Streptococcus mutans compared with nHAp.

According to the results, the addition of boron to nano-hydroxyapatite does not make a significant contribution to increase the regenerative and antibacterial properties of the material.


nano-hydroxyapatite, boron, cytotoxicity, Streptococcus mutans

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.