Is cytokeratin-19 an usefull detection marker of carcinoma cells in the peripheral blood breast cancer cells?

Introduction: Breast cancer is one of the most common neoplasm in women population. In many patients with epithelial tumors, also in breast cancer, the circulating epithelial cells in the peripheral blood in the group of patients with and without metastases were observed. Cytokeratin-19 is a marker which can be use to identification these cells in the peripheral blood.
Objectives:1. The opinion of possibility of utilization sign of cytokeratin-19 expression to detection the presence of cancer cells in the group of patients with breast cancer in the peripheral blood.
2. Examining and the finding among level of gene expression the correlation of cytokeratin-19 and the classic prognostic factors in breast cancer.
3. Opinion of level of cytokeratin-19 expression as a diagnostic and prognostic factors at women with breast cancer.
Material and method: 11 potentially healthy women, 30 women with benign tumors and 30 women with breast cancer before operating treatment were classified to this investigation breast. The detection of epithelial cells from the peripheral blood were held by RT-PCR method.
Results: The analysis of detection the mRNA telomerase from the peripheral blood of examined patients showed, that the results of this research at considerable majority were positive. The difference in frequency of occurrence of positive results were not statistical magnificent.
Conclusions: The diagnostic meaning of presence of the carcinoma cells in the peripheral blood need more investigations.