eISSN: 1897-4252
ISSN: 1731-5530
Kardiochirurgia i Torakochirurgia Polska/Polish Journal of Thoracic and Cardiovascular Surgery
Current issue Archive About the journal Supplements Abstracting and indexing Contact Instructions for authors
SCImago Journal & Country Rank

2/2012
vol. 9
 
Share:
Share:
more
 
 
abstract:

Myocardial infarction in mice – tips and pitfalls on the surgical and echocardiographic techniques

Joanna Śliwka, Bartłomiej Kumaszka, Marcin Garbacz, Sławomir Pakuło, Karolina Nowak, Maciej Domagała, Tomasz Jaźwiec, Mateusz Zandecki, Tomasz Cichoń, Jerzy Nożyński, Ryszard Smolarczyk, Stanisław Szala, Marian Zembala, Michał Zembala

Kardiochirurgia i Torakochirurgia Polska 2012; 2: 243–251
Online publish date: 2012/07/02
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Cardiovascular research on myocardial physiology is often based on animal models of heart ischaemia. The model of left anterior descending (LAD) ligation in the mouse has been widely used to test various experimental hypotheses. However, there are significant discrepancies among studies not only in terms of technical aspects of the model, but more importantly, in early and late outcomes. Moreover, there is no step-by-step guide on how to create the model, avoid difficulties, pitfalls and technical hazards, and thus minimize early and late mortality. This paper is intended to serve as a reference for cardiovascular laboratories wishing to successfully start and maintain a reliable and reproducible model of LAD ligation in mice.
keywords:

myocardial infarct, research animal model

references:
Szymczak E, Lipiec P, Kurpisz M, Krzemińska-Pakuła M, Kasprzak JD. Zaawan-
sowane metody echograficznej oceny funkcji mięśnia sercowego u myszy
z indukowanym zawałem mięśnia sercowego. Pol Prz Kardiol 2009; 11: 189-194.
Tarnavski O, McMullen JR, Schinke M, Nie Q, Kong S, Izumo S Mouse cardiac surgery: comprehensive techniques for the generation of mouse models of human diseases and their application for genomic studies. Physiol Geno-
mics 2004; 16: 349-360.
Takagawa J, Zhang Y, Wong ML, Sievers RE, Kapasi NK, Wang Y, Yeghiazarians Y, Lee RJ, Grossman W, Springer ML Myocardial infarct size measurement in the mouse chronic infarction model: comparison of area- and length-based approaches. J Appl Physiol 2007; 102: 2104-2111.
Roubille F, Combes S, Leal-Sanchez J, Barrère C, Cransac F, Sportouch-Dukhan C, Gahide G, Serre I, Kupfer E, Richard S, Hueber AO, Nargeot J, Piot C,
Barrère-Lemaire S. Myocardial expression of a dominant-negative form of Daxx decreases infarct size and attenuates apoptosis in an in vivo mouse model of ischemia/reperfusion injury. Circulation 2007; 116: 2709-2717.
Redel A, Jazbutyte V, Smul TM, Lange M, Eckle T, Eltzschig H, Roewer N, Kehl F.
Impact of ischemia and reperfusion times on myocardial infarct size in mice in vivo. Exp Biol Med 2008; 233: 84-93.
Lutgens E, Daemen MJ, de Muinck ED, Debets J, Leenders P, Smits JF. Chro-
nic myocardial infarction in the mouse: cardiac structural and functional changes. Cardiovasc Res 1999; 41: 586-593.
Kido M, Du L, Sullivan CC, Li X, Deutsch R, Jamieson SW, Thistlethwai-
te PA. Hypoxia-inducible factor 1-alpha reduces infarction and attenuates progression of cardiac dysfunction after myocardial infarction in the mouse.
J Am Coll Cardiol 2005; 46: 2116-2124.
Hoit BD, Ball N, Walsh RA. Invasive hemodynamics and force-frequency relationships in open- versus closed-chest mice. Am J Physiol 1997; 273 (5 Pt 2):
H2528-2533.
van den Bos EJ, Mees BM, de Waard MC, de Crom R, Duncker DJ. A novel model of cryoinjury-induced myocardial infarction in the mouse: a comparison with coronary artery ligation. Am J Physiol Heart Circ Physiol 2005; 289: H1291-1300.
Ahn D, Cheng L, Moon C, Spurgeon H, Lakatta EG, Talan MI. Induction of myocardial infarcts of a predictable size and location by branch pattern probability-assisted coronary ligation in C57BL/6 mice. Am J Physiol Heart Circ Physiol 2004; 286: H1201-1207.
http://cardiogenomics.med.harvard.edu/projects/p1/education/MI/cvgen_anim.html.
Du XJ, Gao XM, Ramsey D. Surgical methods of inducing transverse aortic stenosis and myocardial infarction in the mouse. Asia Pacific Heart J 1998; 7: 187-192.
Gao XM, Dart AM, Dewar E, Jennings G, Du XJ. Serial echocardiographic assessment of left ventricular dimensions and function after myocardial infarction in mice. Cardiovasc Res 2000; 45: 330-338.
Patten RD, Aronovitz MJ, Deras-Mejia L, Pandian NG, Hanak GG, Smith JJ, Mendelsohn ME, Konstam MA. Ventricular remodeling in a mouse model of myocardial infarction. Am J Physiol 1998; 274 (5 Pt 2): H1812-820.
Yang Z, Berr SS, Gilson WD, Toufektsian MC, French BA. Simultaneous evaluation of infarct size and cardiac function in intact mice by contrast-enhanced cardiac magnetic resonance imaging reveals contractile dysfunction in noninfarcted regions early after myocardial infarction. Circulation 2004; 109: 1161-1167.
Springer ML, Sievers RE, Viswanathan MN, Yee MS, Foster E, Grossman W,
Yeghiazarians Y. Closed-chest cell injections into mouse myocardium guided by high-resolution echocardiography. Am J Physiol Heart Circ Physiol 2005; 289: H1307-1314.
http://jaxmice.jax.org/strain/000664.html.
Zolotareva AG, Kogan ME. Production of experimental occlusive myocardial infarction in mice. Cor Vasa 1978; 20: 308-314.
http://www.aalas.org/.
http://www.iacuc.ucsf.edu/Proc/awMouseFrm.asp.
Buitrago S, Martin TE, Tetens-Woodring J, Belicha-Villanueva A, Wilding GE. Safety and efficacy of various combinations of injectable anesthetics in BALB/c mice. J Am Assoc Lab Anim Sci 2008; 47: 11-17.. Lutz M, Rosenberg M, Kiessling F, Eckstein V, Heger T, Krebs J, Ho AD, Kat-
us HA, Frey N Local injection of stem cell factor (SCF) improves myocardial homing of systemically delivered c-kit + bone marrow-derived stem cells. Cardiovasc Res 2008; 77: 143-150.
Brown RH, Walters DM, Greenberg RS, Mitzner W. A method of endotracheal intubation and pulmonary functional assessment for repeated studies in mice. J Appl Physiol 1999; 87: 2362-2365.
Bialik S, Geenen DL, Sasson IE, Cheng R, Horner JW, Evans SM, Lord EM, Koch CJ, Kitsis RN. Myocyte apoptosis during acute myocardial infarction in the mouse localizes to hypoxic regions but occurs independently of p53. J Clin Invest 1997; 100: 1363-1372.
Li Q, Li B, Wang X, Leri A, Jana KP, Liu Y, Kajstura J, Baserga R, Anversa P. Overexpression of insulin-like growth factor-1 in mice protects from myocyte death after infarction, attenuating ventricular dilation, wall stress, and cardiac hypertrophy. J Clin Invest 1997; 100: 1991-1999.
Bauer M, Cheng S, Jain M, Ngoy S, Theodoropoulos C, Trujillo A, Lin FC, Liao R. Echocardiographic speckle-tracking based strain imaging for rapid cardiovascular phenotyping in mice. Circ Res 2011; 108: 908-916.
Syed F, Diwan A, Hahn HS. Murine echocardiography: a practical approach for phenotyping genetically manipulated and surgically modeled mice.
J Am Soc Echocardiogr 2005; 18: 982-990.
Quick links
© 2019 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe