eISSN: 1897-4252
ISSN: 1731-5530
Kardiochirurgia i Torakochirurgia Polska/Polish Journal of Thoracic and Cardiovascular Surgery
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vol. 9

Myocardial infarction in mice – tips and pitfalls on the surgical and echocardiographic techniques

Joanna Śliwka, Bartłomiej Kumaszka, Marcin Garbacz, Sławomir Pakuło, Karolina Nowak, Maciej Domagała, Tomasz Jaźwiec, Mateusz Zandecki, Tomasz Cichoń, Jerzy Nożyński, Ryszard Smolarczyk, Stanisław Szala, Marian Zembala, Michał Zembala

Kardiochirurgia i Torakochirurgia Polska 2012; 2: 243–251
Online publish date: 2012/07/02
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Cardiovascular research on myocardial physiology is often based on animal models of heart ischaemia. The model of left anterior descending (LAD) ligation in the mouse has been widely used to test various experimental hypotheses. However, there are significant discrepancies among studies not only in terms of technical aspects of the model, but more importantly, in early and late outcomes. Moreover, there is no step-by-step guide on how to create the model, avoid difficulties, pitfalls and technical hazards, and thus minimize early and late mortality. This paper is intended to serve as a reference for cardiovascular laboratories wishing to successfully start and maintain a reliable and reproducible model of LAD ligation in mice.

myocardial infarct, research animal model

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