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Polish Journal of Pathology
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vol. 70
Case report

Spontaneous pathological complete regression of high-grade triple-negative breast cancer with axillary metastasis

Gábor Cserni
Orsolya Serfozo
Éva Ambrózay
László Markó
László Krenács

Institute of Pathology, University of Szeged, Szeged, Hungary
Breast Imaging Unit, Mamma Rt, Kecskemét, Hungary
Departments of Oncology and Surgery, Bács-Kiskun County Teaching Hospital, Kecskemét, Hungary
Laboratory of Tumour Pathology and Molecular Diagnostics, Szeged, Hungary
Pol J Pathol 2019; 70 (2): 139-143
Online publish date: 2019/09/04
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Spontaneous regression of malignant tumours is defined as the disappearance of cancer without any therapy given or following a treatment that is considered inadequate to cure this disease. It is a rare event, estimated to occur in 1 out of 60,000 to 100,000 cases [1], and is more common in some types of tumours (e.g. melanoma) than others. Breast cancer regression was reported in 43/741 cases of spontaneously regressing cancers compiled and summarized by Challis and Stam reviewing the period of 1900-1987 [2], and only a few additional reports have been published since [3 4, 5, 6, 7]. Several mechanisms have been implicated, but most authors associate spontaneous regression with variously activated antitumour immunity [2]. Here, we report on a biopsy-proven breast cancer with axillary metastasis that spontaneously regressed completely in less than 2 months.

Case report

A 72-year-old obese female with type 2 diabetes and hypertension was recalled after attending an opportunistic screening mammography (day 0 of her presented history; Fig. 1A). She appeared 27 days later, when her right breast was perceived as having a 7-mm circumscribed nonpalpable benign lesion with microcalcification in the upper-outer quadrant at the 10 o’clock position, with a physically negative ipsilateral axilla, which on ultrasound (US) showed an enlarged lymph node (13 × 8 mm) with a thickened cortical layer.
In contrast, the upper-outer quadrant of her left breast harboured an ill-defined, spiculated mass with microcalcification (Fig. 1B); this corresponded to a firm mobile palpable mass measuring 16 mm in the greatest dimension on US, located in the two o’clock position, 2 cm from the areola and 6 mm deep from the skin. The left axilla demonstrated a rounded pathological lymph node, which was not present on the screening mammogram (Fig. 1B); this measured 34 × 25 mm on US. 14G needle core biopsy was obtained from the left sided primary tumour, and this resulted in a grade 3 (poorly differentiated) carcinoma with medullary features, also classified as lymphocyte predominant breast cancer with over 60% stromal mononuclear cells [8] (Fig. 2A). The phenotype was triple-negative, i.e. the tumour cells were negative for oestrogen receptors (ER), progesterone receptors (PR), and human epidermal growth factor receptor-2 (HER2), but showed cytokeratin 5 staining and high (about 60%) Ki-67/MIB1 labelling on immunohistochemistry. US-guided fine needle aspiration cytology was done on both axillary lymph nodes described, yielding a negative result on the right side and proving metastatic involvement on the left side (Fig. 2B). Magnetic resonance imaging of the breasts could not be performed due to the patient being oversized for the machine. On the basis of a multidisciplinary team decision, the right sided breast lesion was also targeted with US-guided core needle biopsy on day 40; this resulted in an invasive carcinoma diagnosis (luminal A-like no special type carcinoma, grade X – ungradable due to limited amount of material, but non-high grade; ER and PR highly expressed, HER2-negative, CK5-negative, Ki-67/MIB1 labelling of around 10%, Table I). Performance of bilateral radio-guided and wire-guided wide local excisions plus right sided sentinel lymph node biopsy and left sided axillary lymph node dissection were decided; these were done on day 83 of the presented medical history, 56 days after the left sided biopsies. The left sided lesion was not easy to localise preoperatively (Fig. 1C), but the critical area was confirmed to be removed by specimen mammography (Fig. 1D). The 82-g, dominantly fatty specimen included a 14 × 11-mm tumour bed with no residual carcinoma, but wavy loose fibrotic tissue, and focal aggregates of lymphocytes and calcifications (Fig. 2C). No lymph node metastases were detected, but two lymph nodes also demonstrated signs of regression with a similar wavy loose fibrous tissue (Fig. 2D). Although radio-pathological correlation was present, a repeated breast imaging was performed on day 131; it showed a postoperative status with clips in the excision area, but no signs of residual disease (Fig. 1E). The whole excised breast was investigated histologically, and this showed no residual invasive tumour or tumour bed; however, a low-grade (well-differentiated), ER-positive, HER2-negative, and CK5-negative solid and cribriform ductal carcinoma in situ (DCIS) of 8 mm greatest dimension was found away from the tumour bed. We obtained a careful history of the patient. There have been no changes in food, nor in drugs taken for her diabetes and hypertension. Metformin (1000 mg twice daily), an antidiabetic with reported antitumor activity [9] had been part of her treatment for many years, she was taking it before the diagnosis of breast cancer, during its fast nodal progression (Fig. 1A, B) and its regression (Fig. 1B, C). Spontaneous regression of the grade 3 carcinoma and its axillary metastases was established, and the ipsilateral DCIS identified was considered an incidentally identified different disease. The right sided carcinoma corresponded to a 5-mm carcinoma of no special type, and its single sentinel node was free of metastasis. Adjuvant aromatase inhibitor treatment was initiated. Histological re-appraisal of the primary tumour sample of the regressed carcinoma demonstrated dense peritumoral and intratumoural stromal lymphocytic infiltrate giving the impression that virtually every tumour cell was in direct physical contact with lymphocytes (Fig. 2A, E-H). These lymphocytes were predominantly CD8+/granzyme B+ activated cytotoxic T-cells, and scattered apoptotic tumour cells could also be appreciated (Fig. 2G). Furthermore, the sample demonstrated diffuse programmed cell death ligand 1 (PD-L1) expression in tumour cells and infiltrating immune cells (Fig. 2H), while programmed cell death (PD1) molecules showed up to 50% positivity rate restricted to the infiltrating lymphocytes.


Spontaneous regression of breast cancer is a rare event, and although recognised in the medical literature, it is still a surprising phenomenon. With current interest in antitumour immunity and its stimulation with immunotherapy and immune checkpoint inhibitors, these cases might deserve special attention.
Due to the rarity of the phenomenon of spontaneous regression, case reports and their compilations only collect possible explanations, and the study of the reported single cases is restricted by the lack of sufficient data on a number of newly introduced biomarkers. Immune mechanisms are the most commonly mentioned possible cause of cancer regression, but infectious, hormonal, operative trauma-related, ischaemia/necrosis-related factors have all been mentioned in the literature [2].
Even the rare case reports on spontaneously regressive breast cancer are heterogeneous: they include partial or nearly complete regression [4, 5], and complete regression cases [3, 4]. The preoperative diagnosis of spontaneously regressive breast cancers is also variable, some rely on fine needle aspirates only [5, 7], and a few have a tissue diagnosis by core needle biopsy [3, 6], allowing the assessment of the lymphoid component at diagnosis. The postoperative assessment either showed necrosis with inflammatory cells [5] or, as in the presented case, a fibrotic tumour bed with inflammatory cells [7]. The phenotype of the tumours has been rarely reported, but it seems that oestrogen receptor-positive carcinomas have also regressed spontaneously [5], or on corticosteroid alone [4], although the phenomenon might be more common in triple-negative carcinomas [6] or oestrogen and progesterone receptor negative carcinomas when HER2 was not tested [3]. Our spontaneously regressive case seems to represent a basal-like carcinoma on the basis of the triple-negative phenotype, and diffuse CK5 and p53 expression [10]. In keeping with the presented history, medullary carcinomas (generally triple-negative in phenotype) had better prognosis than carcinomas of no special type of the same grade, and medullary carcinomas with greater amount of lymphoid stroma had better survival than those with fewer mononuclear cells [11], pointing to the role tumour infiltrating lymphocytes may play in antitumour defence.
Both CD4- and CD8-positive subsets of CD3-positive T-cells have been implicated in the genesis of spontaneous regression [5], although natural killer cells were also suggested in another case [4]. The initiation might be related to the biopsy trauma, as suggested by Maillet et al. [6]. Our findings suggest a robust antitumour immune response mediated by activated cytotoxic (killer) T-cells. To the best of our knowledge, our report is the first that reveals strong and diffuse tumoural expression of PD-L1 in spontaneously regressive breast cancer, a molecule that may play a major role in tumour-associated T-cell inhibitory mechanisms [12]. One could assume that such a PD-L1 expression is contrary to spontaneous regression because high PD-L1 expression was found to be associated with poor prognosis [13], but this phenomenon may suggest that the antitumour immune response has the ability to spontaneously overcome inhibitory mechanisms induced by cancerous growth. Our finding of strong and diffuse PD-L1 expression is in keeping with the results obtained on a relatively large tissue microarray study associating PD-L1 expression with oestrogen receptor negativity, progesterone receptor negativity, high histological grade, high Ki67 labelling, and high numbers of tumour infiltrating lymphocytes, CK5 expression, and p53 expression [14]. High PD-L1 labelling is relatively common in triple-negative breast carcinomas, and a better prognosis has been associated with lymphocyte predominant breast carcinomas showing high PD-L1 expression [14], which is also in line with the reported case. However, it remains elusive how the presented tumour managed to overcome the PD-L1 – PD1 axis spontaneously because the results quoted in the previous sentence were gained from tumours of patients who received systemic treatment. The potential role of metformin was considered unlikely due to the drug having been taken even before the progressive phase of the regressing carcinoma.
This work was partly supported by the National Research, Development, and Innovation Office grant number: GINOP-2.3.2-15-2016-00020.
The authors declare no conflict of interest.


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Copyright: © 2019 Polish Association of Pathologists and the Polish Branch of the International Academy of Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (http://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
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