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1/2024
vol. 23 abstract:
Original paper
Utility of expression of 4-hydroxynonenal tested by immunohistochemistry for cervical cancer
Eva Tsoneva
1
,
Polina Dimitrova
2
,
Metodi Metodiev
3
,
Velizar Shivarov
4
,
Mariela Vasileva-Slaveva
5
,
Angel Yordanov
6
,
Stoyan Kostov
7
Menopause Rev 2024; 23(1): 6-13
Online publish date: 2024/03/18
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Introduction
Cervical cancer (CC) is a leading cause of mortality in women around the world, with the highest incidence rate still being in developing countries. The most common aetiological factor is infection with high-risk human papilloma virus viral strains. Oxidative stress through generation of reactive oxygen species leads to lipid peroxidation and DNA damage. Studies show that reactive lipid electrophiles such as 4-hydroxynonenal (4-HNE) produced in the process play an important role in cancer signalling pathways and are a good biomarker for oxidative stress. We aim to investigate the prognostic role of 4-HNE as a biomarker for oxidative stress in patients in early and advanced stages of CC measured by immunohistochemistry. Material and methods This is a retrospective study of 69 patients treated at our Department of Oncogynaecology. Paraffin embedded tumour tissues were immunohistochemically tested for the levels of expression of 4-HNE. The results for H-score, Allred score, and combined score were investigated for association with tumour size, lymph node status, and FIGO stage. Results 4-hydroxynonenal showed higher expression in more advanced stages of CC and in cases with involved lymph nodes. Tumour size was not associated with the levels of 4-HNE. Conclusions To best of our knowledge, this is the first study to use immunohistochemistry to examine the expression of 4-HNE as a prognostic factor in CC. The 3 score systems showed similar results. The pattern of 4-HNE histological appearance is dependent on the histological origin of cancer and is not universal. keywords:
4-HNE, cervical cancer, ROS, lipid metabolism, oxidative stress |
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