eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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1/2011
vol. 36
 
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abstract:

Experimental immunology
Colonization of prospective heart graft with recipient bone marrow dendritic cells prior to transplantation does not prolong graft survival

Michał Maksymowicz
,
Waldemar L. Olszewski
,
Bożenna Interewicz

(Centr Eur J Immunol 2011; 36 (1): 9-14)
Online publish date: 2011/03/31
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Dendritic cells (DC) have been implicated in the induction of tolerance. We studied the tolerogenic effect of recipient DCs on the heart allografts. The prospective grafts were populated prior to transplantation by recipient bone marrow DCs and subsequently transplanted to the recipient. Briefly, LEW rats were depopulated from own DCs by irradiation. Then, they received vascularized bone marrow graft from BN rats (in transplanted hind-limb). Subsequently, LEW hearts and skin with BN bone marrow derived DCs were transplanted to normal and tacrolimus-treated BN rats. Replacement of LEW donor heart and skin with either of mature or immature BN DCs did not prolong graft survival in BN recipient. The intragraft microchimerism did not mitigate the allogeneic rejection reaction. To the contrary, the BN donor DCs repopulating LEW heart or skin initiated the against-recipient reaction in the prospective graft already during the repopulation process. Immunosuppression with Tacrolimus prolonged the BN DC-repopulated LEW grafts survival as long as it was administered. Weaning from immunosuppression was followed by rejection of heart grafts after 7-14 days, whereas those of skin after 3 days. LEW hearts populated with BN DCs and transplanted back to normal LEW rats underwent chronic rejection and stopped contracting 2 to 12 weeks after grafting. Taken together, replacement of donor hearts with recipient DC did not result in prolongation of graft survival. It initiated the alloreaction in the graft already during repopulation. Recipient DC retained their immunogenic properties also when the graft was returned back to the donor.
keywords:

: transplantation, dendritic cells, tolerance, bone marrow, heart


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