eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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1/2011
vol. 36
 
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abstract:

Experimental immunology
Modulatory effect of Echinacea pallida on cellular immunity and angiogenesis in mice

Ewa Skopińska-Różewska
,
Aleksander Wasiutyński
,
Ewa Sommer
,
Piotr Skopiński
,
Krzysztof Pastewka
,
Robert Zdanowski
,
Janusz Bany

(Centr Eur J Immunol 2011; 36 (1): 18-23)
Online publish date: 2011/03/31
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The aim of this work was to evaluate the in vivo effect of commercial preparation of Echinacea pallida (Lymphozil) on the in vitro chemokinetic activity of mouse splenic lymphocytes in tissue culture, and on their in vivo immunological response against foreign histocompatibility antigens in local graft-versus-host reaction (lymphocyte-induced angiogenesis, LIA). The effects of feeding mice E. pallida on metabolic activity of blood granulocytes (chemiluminescence test) and on the bacterial infection (Pseudomonas aeruginosa) were also investigated. The ability of E. pallida to influence neovascular response induced in mice skin by grafting of human kidney cancer cells (tumor- induced angiogenesis, TIA test) was also studied. We have found significant stimulation of LIA activity of splenic lymphocytes collected from animals fed 0.02 mg daily dose of E. pallida, no effect in group fed 0.2 mg and significant inhibition in group fed the highest 2 mg dose. In the case of chemokinetic activity (in vitro migration of cells in culture) the highest stimulatory effect was obtained when cells were obtained from animals fed 0.02 mg daily dose. Higher doses were also stimulatory but this effect was less pronounced.

In experiments with bacterial infection mice were fed for 5 days 0.08 mg daily dose of E. pallida and this treatment resulted in highly significant diminution of number of bacterial colonies in liver in comparison to the control.

Blood granulocytes metabolic activity was unaffected in mice fed 0.02 and 0.2 mg daily dose, and significantly lower in mice fed highest, 2 mg dose.

In experiments with kidney tumor all three doses of E. pallida reduced the neovascular response originated in mice skin after transplantation of cancer cells.
keywords:

mice, Echinacea pallida, immunological angiogenesis, tumor angiogenesis, granulocytes chemiluminescence, splenocytes chemokinesis, bacterial infection


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