eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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2/2012
vol. 37
 
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abstract:

Experimental immunology
The effect of sterilization by irradiation of human pericardium and skin frozen tissues on their ability to influence the proliferation of endothelial cells in vitro

Robert Zdanowski
,
Wanda Stankiewicz
,
Artur Kamiński
,
Tomasz Grzela
,
Piotr Skopiński
,
Sławomir Lewicki
,
Ewa Skopińska-Różewska

(Centr Eur J Immunol 2012; 37 (2): 119-122)
Online publish date: 2012/05/22
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The delayed wound healing is considered as important medical, social and economical problem. The effective wound management is one of major goals in health system strategy. Various dressing materials were used for this purpose among them skin and pericardium. The aim of the present paper was to compare the effect of these two materials, frozen and radiation-sterilized or non-sterilized, on the proliferation of HECa10 endothelial cells (EC) in vitro.

The influence of tissue samples on EC proliferation was evaluated with the Alamar Blue (AB) reagent, allowing the evaluation of viability and metabolic activity of cells.

Stimulation of EC proliferation was obtained for all used materials except sterilized pericardium, in comparison to the control cultures containing 10% of FBS (fetal bovine serum). No difference was observed between sterilized and non-sterilized skin.
keywords:

biological dressings, pericardium, skin, endothelial cells, proliferation

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