|
Current issue
Archive
Online First
About the journal
Editorial board
Abstracting and indexing
Subscription
Contact
Ethical standards and procedures
Special Issues
Instructions for authors
Publication charge
Editorial System
Submit your Manuscript
Open access 
|
1/2002
vol. 1 abstract:
C-erbB2 (Her-2/neu) gene amplification in women with breast cancer – initial studies
Andrzej Kulig
,
Beata Smolarz
,
Elżbieta Kozłowska
,
Hanna Romanowicz-Makowska
,
Marek Zadrożny
(Prz Menopauz 2002; 1: 11–14)
Online publish date: 2004/03/03
View
full text
Get citation
ENW EndNote
BIB JabRef, Mendeley
RIS Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
Background. The c-erbB2 (Her2/neu) oncogene is located on the long arm of chromosome 7, encoding for a 185 kD transmembrane glycoprotein p185 with tyrosine kinase receptor function. Several studies have shown that overexpression of c-erbB2 (Her-2/neu) gene has been associated with breast cancer appearance. The aim of the present study was to investigate the relationship between amplification of c-erbB2 gene and the appearance of breast cancer in menopausal women.
Materials and methods. Paraffin embedded tumour tissue were obtained from patients with breast cancer (n=20). Blood samples from age matched healthy women (n=20) served as control. The amplification of c-erbB2 gene was determined by PCR reaction using the allele specific primers. Results. The amplification of c-erbB2 gene was significantly higher in patients compared as control (p<0.05). Conclusion. The results suggest that amplification of c-erbB2 gene may be linked with appearance of breast cancer in menopausal women. keywords:
c-erbB2 (Her-2/neu) gene, amplification, breast carcinoma, menopause, PCR |
 |
