eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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SCImago Journal & Country Rank
2/2013
vol. 38
 
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abstract:

Clinical immunology
The metabolic activity of neutrophils measured with a chemiluminescence assay in young patients with type 1 diabetes depending on the disease duration

Magdalena Kaczmarek
,
Zdzisława Iwanicka
,
Adam Jankowski
,
Aleksandra Lewandowicz-Uszyńska

(Centr Eur J Immunol 2013; 38 (2): 237-242)
Online publish date: 2013/07/08
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The study deals with the estimation of the metabolic activity of neutrophils measured with a chemiluminescence assay in children with type I diabetes depending on the disease duration, compared to the control group. The study involved 87 children with type I diabetes aged 4 to 21 years (52 girls, 35 boys). Patients were divided into four subgroups depending on the disease duration: subgroup I – the disease duration up to 12 months (35 children), subgroup II – the disease duration up to 5 years (28 children), subgroup III – the disease duration up to 10 years (12 children), and subgroup IV – the disease duration over 10 years (12 children). The control group was composed of 23 persons – healthy volunteers aged 20 to 25 years (mean age 23 years). The metabolic activity of neutrophils was determined in a luminol-dependent chemiluminescence assay. The spontaneous and stimulated chemiluminescence of

neutrophils (fMLP, opsonised Zymosan, PMA) was tested on a 96-well microplate (LB96P-WMP).

The chemiluminescence assay was conducted using a luminometer (MicroLumat LB 96P of E&G BARTHOLD). The results have been written in RLU units (Relative Light Units/s) as the maximum value of chemiluminescence (CLmax). The test showed statistically significantly lower values of spontaneous and stimulated neutrophil chemiluminescence in all subgroups, compared to the control group. The lowest levels of neutrophil CL were in subgroup III (neutrophils stimulated with fMLP and PMA) and also in children with newly detected diabetes (Zymosan-stimulated neutrophils).
keywords:

diabetes, children, neutrophil chemiluminescence, disease duration

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