eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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vol. 43
Experimental immunology

Duck IL-2 promoter cloning and the effects of methylation status on mRNA levels in immune tissues

Tao Zhang
Hehe Liu
Tianyu Zhou
Jun Luo
Jiwen Wang
Chunchun Han
Jiwei Hu
Qianfeng Wu
Yushi Wang

(Centr Eur J Immunol 2018; 43 (4): 389-398)
Online publish date: 2018/12/31
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Interleukin 2 (IL-2), a cytokine, plays an important role in animal immune systems. To investigate the influences of epigenetic modifications on transcription of the duck IL-2 gene, the promoter region of the duck IL-2 gene was cloned. Then, the DNA methylation status of the IL-2 gene promoter (-1337 bp/-924 bp) in immune tissues of ducks was determined using the Sequenom Mass Array methylation technique, and their corresponding expression levels were determined using real-time PCR. The results showed that 2850 bp of the duck IL-2 gene promoter region were obtained. There was one CpG island (-1231 bp/-902 bp) in which 11 CpG sites were distributed. The CpG1 and CpG2 sites are located between the binding sites of NFAT and AP-1, and they had higher homology methylation patterns in different individuals and tissues. The methylation frequencies of 28.5% CpG sites showed negative correlations with the expression levels of the IL-2 mRNA, whereas 71.5% showed positive correlations. These results indicate that the transcription of duck IL-2 may be distinct from that of mammals. CpG1 (-1284 bp) and CpG2 (-1264 bp) in the duck IL-2 promoter showed a higher homology of methylation patterns, indicating a similar regulatory effect on their gene expression, and these CpG sites may be essential for the regulation of transcription of duck IL-2. The methylation pattern of the IL-2 gene promoter in duck was tissue specific.

CpG, duck, DNA methylation, IL-2, mRNA expression, promoter

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