eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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1/2021
vol. 46
 
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abstract:
Experimental immunology

Effect of acute isooxic hypercapnia on oxidative activity of systemic neutrophils in endotoxemic rabbits

Hanna Billert
1
,
Ewa Bednarek
1
,
Krzysztof Kusza
2
,
Mirosław Ponichter
1
,
Maciej Kurpisz
3

1.
Department of Experimental Anaesthesiology, Chair of Anaesthesiology and Intensive Therapy, Poznan University of Medical Sciences, Poznan, Poland
2.
Clinics of Anaesthesiology and Intensive Therapy, Chair of Anaesthesiology and Intensive Therapy, Poznan University of Medical Sciences, Poznan, Poland
3.
Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland
Cent Eur J Immunol 2021; 46 (1): 47-53
Online publish date: 2021/04/18
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Introduction
Whether carbon dioxide (CO2) affects systemic oxidative phenomena under conditions of endotoxemia is not sufficiently clarified. The study aimed to assess the impact of moderate acute hypercapnia on the respiratory burst of circulating neutrophils in mechanically ventilated endotoxemic rabbits.

Material and methods
Twenty-four endotoxemic rabbits were mechanically ventilated with standard or CO2‑enriched gas mixture in order to obtain isooxic hypercapnia. At a baseline point and following 180 min of hypercapnic ventilation, luminol-dependent chemiluminescence (CL) of circulating neutrophils and serum 2-thiobarbituric acid reactive substance (TBARS) concentrations were measured. Throughout the study, leukocyte and neutrophil counts, pH status, circulatory parameters and body temperature were also assessed.

Results
Following 180 min of hypercapnic ventilation, opsonized zymosan (OZ)-stimulated neutrophils showed lower CL vs. the control group (p = 0.004). Other parameters studied were not affected.

Conclusions
Short-term isooxic hypercapnia in endotoxemic rabbits preserves circulating neutrophil count pattern and reactive oxygen species (ROS) generation, but it may reduce phagocytosis.

keywords:

endotoxemia, hypercapnia, respiratory burst, circulating neutrophils

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