Evaluation of selected immunological parameters and the concentration of vitamin D in children with asthma. Case-control study

Wawrzyniak A, Lipińska-Opałka A, Zdanowski R, Lewicki S, Murawski P, Kalicki B. Evaluation of selected immunological parameters and the concentration of vitamin D in children with asthma. Case-control study. Central European Journal of Immunology. 2017;42(1):101-106. doi:10.5114/ceji.2017.67323.

APA

Wawrzyniak, A., Lipińska-Opałka, A., Zdanowski, R., Lewicki, S., Murawski, P.,  & Kalicki, B. (2017). Evaluation of selected immunological parameters and the concentration of vitamin D in children with asthma. Case-control study. Central European Journal of Immunology, 42(1), 101-106. https://doi.org/10.5114/ceji.2017.67323

Chicago

Wawrzyniak, Agata, Agnieszka Lipińska-Opałka, Robert Zdanowski, Sławomir Lewicki, Piotr Murawski,  and Bolesław Kalicki. 2017. "Evaluation of selected immunological parameters and the concentration of vitamin D in children with asthma. Case-control study". Central European Journal of Immunology 42 (1): 101-106. doi:10.5114/ceji.2017.67323.

Harvard

Wawrzyniak, A., Lipińska-Opałka, A., Zdanowski, R., Lewicki, S., Murawski, P.,  and Kalicki, B. (2017). Evaluation of selected immunological parameters and the concentration of vitamin D in children with asthma. Case-control study. Central European Journal of Immunology, 42(1), pp.101-106. https://doi.org/10.5114/ceji.2017.67323

MLA

Wawrzyniak, Agata et al. "Evaluation of selected immunological parameters and the concentration of vitamin D in children with asthma. Case-control study." Central European Journal of Immunology, vol. 42, no. 1, 2017, pp. 101-106. doi:10.5114/ceji.2017.67323.

Vancouver

Wawrzyniak A, Lipińska-Opałka A, Zdanowski R, Lewicki S, Murawski P, Kalicki B. Evaluation of selected immunological parameters and the concentration of vitamin D in children with asthma. Case-control study. Central European Journal of Immunology. 2017;42(1):101-106. doi:10.5114/ceji.2017.67323.

PlumX metrics:

Due to the increased incidence of allergic diseases and emerging effects of unsatisfactory control of asthma, new mechanisms for supervising the immune system should be searched. The aim of the study was to analyze the percentage of CD3, CD4, CD8, CD19, CD16/56, NKT, CD3 anti-HLADR3 and Foxp3 regulatory lymphocytes in patients with asthma. Additionally the correlation between immune parameters, severity of asthma and serum concentration of vitamin D was performed. 25 children diagnosed with asthma were enrolled. Disease severity was assessed with the Asthma Control Test (ACT) and spirometry. The control group consisted of 15 healthy children. Venous blood from each patient was collected on EDTA or on “clott”. Phenotypes of lymphocytes were evaluated by flow cytometry. Vitamin D concentration was assessed by chemiluminescent immunoassay (CLIA) technology. There was a significant decrease in the percentage of T regulatory cells (p < 0.006) in children with asthma compared to the control group. There were no significant differences in the other investigated immunological parameters. In addition, in asthma group statistically significant decreased of vitamin D concentration (p < 0.04) was observed. There were also no significant correlations between vitamin D3 concentration and the course of asthma or percentage of regulatory cells. The results confirmed the role of regulatory T cells in the pathogenesis of asthma. Effects of vitamin D on the severity of the disease has not been proven.

keywords:

Evaluation of selected immunological parameters and the concentration of vitamin D in children with asthma. Case-control study

Agata Wawrzyniak , Agnieszka Lipińska-Opałka , Robert Zdanowski , Sławomir Lewicki , Piotr Murawski , Bolesław Kalicki

asthma, regulatory T cells (FoxP3), flow cytometry, vitamin D

Agata Wawrzyniak

,

Agnieszka Lipińska-Opałka

,

Robert Zdanowski

,

Sławomir Lewicki

,

Piotr Murawski

,

Bolesław Kalicki