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4/2012
vol. 37
abstract:
Experimental immunology
Human Hsp60 could be a possible target of immune response triggered by Hsp60 of Salmonella Enteritidis – a preliminary study
Bożena Dera-Tomaszewska
,
(Centr Eur J Immunol 2012; 37 (4): 307-313)
Online publish date: 2013/02/10
Hsp60 of Salmonella Enteritidis appears to be involved in pathogenesis of infectious processes and host immune responses. Because of the similarities between microbial and human Hsps, a humoral response against microbial Hsps may be destructive for the host due to antigen mimicry leading to an autoimmune response. We have performed a preliminary study to investigate whether the homology between Hsp60 of Salmonella Enteritidis and human Hsp60 really exists. ELISA tests were done with success to show that polyclonal antibodies developed against Hsp60 of Salmonella Enteritidis recognize and cross-react with human Hsp60 and one of its seven synthetic peptides used in the study. It was revealed that the antigenic epitopes, which both the proteins have in common, are located between 409 and 424 amino acid residues of human Hsp60 molecule, and are determined by the amino acid sequence of human Hsp60 (409-424) synthetic peptide, the same sequence which was found by other authors to be an immunodominant epitope in patients with acute coronary syndromes. The results show the presence of an immunological and sequence similarity between bacterial Hsp60 and its human counterpart, and suggest that human Hsp60 could be a possible target of immune response triggered by Hsp60
of Salmonella Enteritidis. Salmonella Enteritidis Hsp60 might be potentially involved in autoimmune mechanisms operating in humans. This conclusion must be considered preliminary and hypothesis-generating than hypothesis-proving.
keywords:
Hsp60, Salmonella Enteritidis, Hsps homology, antigenic mimicry, immunological similarity
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