Experimental immunology<br>Influence of nonselective and selective phosphodiesterase inhibitors on cAMP levels in lymphocytes after a single administration in mice

Marianna Szczypka; Bożena Obmińska-Mrukowicz

doi:10.5114/ceji.2012.30793

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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3/2012
vol. 37

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abstract:

Experimental immunology
Influence of nonselective and selective phosphodiesterase inhibitors on cAMP levels in lymphocytes after a single administration in mice

Marianna Szczypka

,

Bożena Obmińska-Mrukowicz

(Centr Eur J Immunol 2012; 37 (3): 200-203)

DOI: https://doi.org/10.5114/ceji.2012.30793

Online publish date: 2012/10/27

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The cyclic adenosine 3',5'-monophosphate (cAMP) is an important factor that modulates the activity of immune cells, including lymphocytes. The PDE inhibitors were administered once: aminophylline, a nonselective PDE inhibitor, at a dose of 20 mg/kg i.m., milrinone, a selective PDE3 inhibitor, at a dose of 1 mg/kg i.m., and sildenafil, selective PDE5 inhibitor, at a dose of 1 mg/kg p.o.

The purpose of this study was to determine the effect of the PDE inhibitors under investigation of cAMP levels in the lysates of lymphocytes obtained from thymus, spleen and mesenteric lymph nodes.

It was found that aminophylline, milrinone or sildenafil did not change the cAMP levels in thymocyte lysates. These drugs induced an increase in the levels of cAMP in the lysates of splenocytes. Administration of aminophylline did not influence the cAMP levels in the lysates of mesenteric lymph node lymphocytes. However, a decrease in the cAMP concentration in mesenteric lymph node cells was observed after a single administration of the selective PDE inhibitors: milrinone or sildenafil.

Aminophylline, milrinone and sildenafil had no impact on the cAMP level in lymphocytes from thymus. However, the drugs under investigation changed the cAMP content in lymphocytes from peripheral lymphatic organs. These effects depended on the type of a drug and its selectivity for PDE isozymes.

keywords:

Experimental immunologyInfluence of nonselective and selective phosphodiesterase inhibitors on cAMP levels in lymphocytes after a single administration in mice

Marianna Szczypka , Bożena Obmińska-Mrukowicz

cAMP level, lymphocytes, aminophylline, milrinone, sildenafil, mice

Experimental immunology
Influence of nonselective and selective phosphodiesterase inhibitors on cAMP levels in lymphocytes after a single administration in mice

Marianna Szczypka

,

Bożena Obmińska-Mrukowicz