eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
SCImago Journal & Country Rank

vol. 33

Experimental immunology
Mast cell involvement in zymosan-induced peritonitis in C57Bl/6 mice

Elżbieta Kołaczkowska
Bernd Arnold
Barbara Płytycz

(Centr Eur J Immunol 2008; 33 (3): 91-97)
Online publish date: 2008/05/05
View full text
Get citation
JabRef, Mendeley
Papers, Reference Manager, RefWorks, Zotero
We have shown previously that mast cells (MC) and MC-derived mediators are crucial for early stages of zymosan-induced peritonitis in Swiss and Balb/c mice. Present results revealed the same phenomena in males of C57Bl/6 mice as in the MC-depleted animals (by application of a compound 48/80) the intraperitoneal accumulation of exudatory fluid and cells, measured at 0.5, 6 and 24 hours after zymosan injection, was significantly diminished. In particular, an early vascular permeability and PGE2 content at 30 min of peritonitis, and exudatory leukocyte numbers and MCP-1 content at 6 hours of peritonitis were significantly lower in MC-deprived mice then in those with normal MC population. The peritoneal cavity of intact C57Bl/6 males contains less then 1% of mast cells defined as cells filled with metachromatically stained granules (counted in a haemocytometer) and those with MC-specific
c-kit receptors (determined both by quantitative PCR at mRNA level and by flow cytometry on cell surface). After zymosan injection numbers of cells with metachromatically-stained granules significantly decrease reaching minimum at 6 hours of peritonitis with a parallel significant increase of c-kit positive MCs. These contradictory results might be explained by zymosan-induced degranulation of mast cells and concomitant influx of newly arriving mast cells through the omental milky spots and/or in situ MC proliferation.

mast cells, peritonitis, peritoneal inflammation, vasopermeability, PGE2, MCP-1

Quick links
© 2019 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe