eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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2/2014
vol. 39
 
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abstract:

Experimental immunology
Molecular cloning and sequencing analysis of the interferon receptor (IFNAR-1) from Columba livia

Chao Li
,
Wei Shan Chang

(Centr Eur J Immunol 2014; 39 (2): 159–164)
DOI: https://doi.org/10.5114/ceji.2014.43715
Online publish date: 2014/06/27
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Objective: Partial sequence cloning of interferon receptor (IFNAR-1) of Columba livia.

Material and methods: In order to obtain a certain length (630 bp) of gene, a pair of primers was designed according to the conserved nucleotide sequence of Gallus (EU477527.1) and Taeniopygia guttata (XM_002189232.1) IFNAR-1 gene fragment that was published by GenBank. Special primers were designed by the Race method to amplify the 3’terminal cDNA.

Results: The Columba livia IFNAR-1 displayed 88.5%, 80.5% and 73.8% nucleotide identity to Falco peregrinus, Gallus and Taeniopygia guttata, respectively. Phylogenetic analysis of the IFNAR1 gene showed that the relationship of Columba livia, Falco peregrinus and chicken had high homology.

Conclusions: We successfully obtained a Columba livia IFNAR-1 gene partial sequence. Analysis of the genetic tree showed that the relationship of Columba livia and Falco peregrinus IFNAR-1 had high homology. This result can be used as reference for further research and practical application.
keywords:

Columba livia, IFNAR-1, gene cloning, gene sequence analysis

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