Experimental immunology<br>Paeoniflorin reduced BLP-induced inflammatory response by inhibiting the NF-κB signal transduction in pathway THP-1 cells

Qian Zhang; Jing Zhou; Min Huang; Liqing Bi; Suming Zhou

doi:10.5114/ceji.2014.47729

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors

Editorial System

Submit your Manuscript

4/2014
vol. 39

Send email

Copy url:

abstract:

Experimental immunology
Paeoniflorin reduced BLP-induced inflammatory response by inhibiting the NF-κB signal transduction in pathway THP-1 cells

Qian Zhang

,

Jing Zhou

,

Min Huang

,

Liqing Bi

,

Suming Zhou

(Centr Eur J Immunol 2014; 39 (4): 461-467)

DOI: https://doi.org/10.5114/ceji.2014.47729

Online publish date: 2014/12/15

View full text Get citation

PlumX metrics:

Sepsis is a severe illness in which the bloodstream is overwhelmed by bacteria. Despite effective antibiotic treatment, the mortality of septic shock remains high. In this study, we examined a potential usage of paeoniflorin, anti-inflammatory component for the treatment of sepsis. We established an inflammatory cell line by stimulating human THP-1 cell line with bacterial lipoprotein (BLP), which resulted in an activation of nuclear factor κB (NF-κB) p65 dependent-signal pathway, and in consequence, an increase in tumor necrosis factor α (TNF-α) and interleukin (IL)-6 expression. With this model, we studied the effect of paeoniflorin on the expression of NF-κB and Toll-like receptor 2 (TLR2) mediated signal transduction. Our data indicated that paeoniflorin directly inhibited activation of NF-κB p65, thereby reduced the expression of TNF-α and IL-6 in the BLP stimulated THP-1 cells. Paeoniflorin was also found to inhibit IκB phosphorylation and degradation. However, no significant differences in TLR2 and myeloid differentiation factor 88 (MyD88) expression were observed; therefore, these signaling molecules may not have much anti-inflammatory effect in our cellular model. As such, our current study provided a molecular base for the potential use of paeoniflorin in therapeutic treatment of sepsis induced by bacterial lipoprotein.

keywords:

Experimental immunologyPaeoniflorin reduced BLP-induced inflammatory response by inhibiting the NF-κB signal transduction in pathway THP-1 cells

Qian Zhang , Jing Zhou , Min Huang , Liqing Bi , Suming Zhou

paeoniflorin, bacterial lipoprotein, Toll-like receptor, and TNF-α

Experimental immunology
Paeoniflorin reduced BLP-induced inflammatory response by inhibiting the NF-κB signal transduction in pathway THP-1 cells

Qian Zhang

,

Jing Zhou

,

Min Huang

,

Liqing Bi

,

Suming Zhou