eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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2/2013
vol. 38
 
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abstract:

Experimental immunology
Relationship between Yersinia enterocolitica antibody level and bacterial shedding after challenge in previously immunized pigs

Aleksandra Platt-Samoraj
,
Wojciech Szweda
,
Andrzej K. Siwicki
,
Zbigniew Procajło
,
Elżbieta Mikulska-Skupień
,
Agata Bancerz-Kisiel
,
Anna Szczerba-Turek
,
Kinga Syczyło

(Centr Eur J Immunol 2013; 38 (2): 203-207)
DOI: https://doi.org/10.5114/ceji.2013.35216
Online publish date: 2013/07/08
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Asymptomatic carriers of Yersinia enterocolitica are often found in pigs which are the main reservoir and source of human infection. Carrying of that bacteria is usually connected with a long-term shedding and microbiological contamination of the environment. Thus, the aim of this study was to determine the effect of immunization with a suspension of selected strains of Y. enterocolitica on the duration of pathogen shedding in experimentally infected pigs.

Fifteen pigs were divided into three groups, two experimental and one control. Immunizing suspension was administered subcutaneously in doses of 2 ml (group I) and 5 ml (group II) with the density of 2.7 × 109 CFU/ml of formaldehyde inactivated cells suspended in phosphate-buffered saline in two injections within an interval of two weeks. Pigs were experimentally infected with a 10 ml dose of a pathogenic strain of

Y. enterocolitica O:3 with the density of 2.7 × 109 CFU/ml two weeks after immunization.

Subcutaneous immunization stimulated higher antibody levels in group II, which was administered a higher inoculant dose of 5 ml suspension with the density of 2.7 × 109 CFU/ml. In this group, shedding was not reported in two out of five pigs, and the period of pathogen excretion was shorter in comparison with group I and the control group. Experimental immunization against infections caused by Y. enterocolitica did not prevent pathogen shedding, but merely limited the intensity and duration of bacterial excretion.
keywords:

Yersinia enterocolitica, immunization, challenge

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