eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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2/2014
vol. 39
 
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abstract:

Experimental immunology
The effect of interleukin 17 and Toll-like receptor 2 on CD11b expression and apoptosis of neutrophils in zymosan-induced arthritis and paw oedema

Viktoriya Milanova
,
Nina Ivanovska
,
Petya Dimitrova

(Centr Eur J Immunol 2014; 39 (2): 131–141)
Online publish date: 2014/06/27
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We investigated the effect of interleukin (IL)-17 on Ly6G+ cell apoptosis in zymosan-induced arthritis (ZIA) and oedema (ZIO). Zymosan injection at the ankle joint caused swelling and coincided with histological joint alterations and IL-17A expression in areas with cell infiltrates. Flow cytometry of blood demonstrated increased frequencies of Ly6G+CD11b+ cells and their decreased apoptosis in ZIA. Annexin V+ neutrophils had lower CD11b expression, unlike Annexin V– cells. Cell survival for 12 hours was affected neither by IL-17 nor by zymosan alone, while both stimuli diminished Annexin V+ cell frequencies and up-regulated CD11b on Annexin V– cells. Interleukin 17 antagonised to the effects of zymosan in 24-hour cultures. The administration of IL-17 in ZIO increased paw thickness, enlarged the blood Ly6G+ pool, elevated CD11b expression and decreased apoptosis. We suggest that altered neutrophil apoptosis in arthritis can be overcome by anti-IL-17 therapy combined with an inhibition of Toll-like receptor (TLR) 2 and CD11b signalling.
keywords:

neutrophils, CD11b, interleukin 17, neutrophils, TLR2 ligand, paw oedema

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