eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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SCImago Journal & Country Rank
2/2022
vol. 47
 
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abstract:
Experimental immunology

Generation of high cross-presentation ability human dendritic cells by combination of interleukin 4, interferon β and GM-CSF

Yihua Pi
1
,
Yifang Li
2
,
Rongyi Liang
1, 3
,
Jian Xiao
1, 3
,
Jing Leng
1, 3
,
Lifeng Zhang
1, 3

1.
Guangxi University of Chinese Medicine, China
2.
The Second People’s Hospital of Hunan Province, China
3.
Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine, China
Cent Eur J Immunol 2022; 47 (2): 125-138
Online publish date: 2022/07/04
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Dendritic cell (DC)-based immunotherapies have been utilized for the treatment of numerous diseases. However, the conventional generation strategies of DCs in vitro require 7 days and these DCs showed an unsatisfactory function, which prompted us to explore new approaches. We found that in vitro culture of human CD14+ cells, in the medium containing granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-4, as well as interferon  (IFN-) for 48 h, followed by the maturation stimuli of IL-1 and poly I:C for another 24 h can be differentiated into high cross-presentation ability DCs (G4B-DCs). These DCs express high levels of CD11c, CD86, and HLA-DR, producing a high level of tumor necrosis factor  (TNF-). Of note, compared with the conventional DCs, G4B-DCs showed a higher ability to promote allogeneic naïve CD4+ T cell and CD8+ T cell proliferation and interferon (IFN)- production. These DCs also have the remarkable ability to induce Flu-M1-specific CD8+ T cells. In addition, we found that these G4B-DCs express partially the cDC1 phenotype. These data indicate that G4B-DC is unique and may provide a relatively rapid alternative method for potential clinical use.
keywords:

IL-4, dendritic cell, cDC1, cross-presentation, IFN-b

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