This study aimed to determine the association between pre-competition perceived player wellbeing measures and subsequent relative and peak running performance of developmental youth female soccer players (n = 15, age: 16±1 years). Total distance (TD), high-speed (> 3.5 m/s) (HSRD) and very high-speed (> 5.3 m/s) running (VHSRD) were expressed using 1-, 2- and 5-minute epochs and relative (per minute) calculations. Fatigue, sleep quality, upper and lower-body muscle soreness, stress, and mood wellbeing measures were collected via a self-reported questionnaire (1–5 Likert scale). Menstrual cycle phase was collected via a calendar-based countback method. Results demonstrated that reductions in stress was associated with decreased relative and peak TD in all epochs (p = 0.008–0.040), relative and peak HSRD (p = 0.006–0.039) in 2- and 5-minute epochs as well as VHSRD in 2-minute epochs (p = 0.026). For example, a one-point reduction of ‘normal’ to ‘relaxed’ is associated with a decrease of 7 m/min in peak TD for 1-minute epochs. One-point increase in fatigue (e.g., ‘normal’ to ‘more tired than normal’) displayed a decrease of 7 m/min peak TD for 2-minute (p = 0.048) and 9 m/min for 5-minute (p = 0.007) rolling epochs. Likewise, one-point increase in lower-body muscle-soreness (e.g., ‘normal’ to ‘increase in soreness/tightness’) was associated with a reduction of 6 m/min peak VHSRD for 1-minute epochs (p = 0.034). Results suggest that perceived player wellbeing can influence running performance. However, the magnitude of the change in player wellbeing should be considered in a practical sense.