eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank

2/2018
vol. 43
 
Share:
Share:
more
 
 
abstract:
Experimental immunology

In search of chondrocyte-specific antigen

Anna Osiecka-Iwan, Piotr Skopiński, Dorota M. Radomska-Leśniewska, Anna Hyc

(Centr Eur Immunol 2018; 43 (2): 132-138)
Online publish date: 2018/06/30
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
The purpose of this work was to establish, whether rat chondrocyte associated antigen, transmembrane Tmp21 protein belonging to the p24 protein family may immunize rats and thus be included into the panel of immunogens potentially involved in cartilage pathology. For immunization of rats extract from cultured chondrocytes containing surface chondrocyte proteins suspended in incomplete Freund’s adjuvant was used. Control animals were injected with incomplete Freund’s adjuvant without chondrocyte extract. Morphological observations indicated that both in control and experimental animals occurred subperiosteal resorption of bone, suggesting that it arised as the response to adjuvant. In trachea, however, resorption of cartilage and inflammatory changes in the respiratory epithelium and lamina propria were present only in animals exposed to antigen. Unexpectedly, sera from immunized rats strongly reacted with other antigen, which we were able to identify by Western blot and protein sequencing as cartilage oligomeric matrix protein (COMP). COMP is attached to chondrocyte membrane by integrins and its presence in chondrocyte extract is not surprising. Antibody response to COMP raises a question whether the observed changes in tracheal cartilage and epithelium represent anti-COMP reaction or were caused by some other, no specified factors. COMP is used as the marker of osteoarthritis progression, but its role in polychondritis, cartilage pathology involving i.a. tracheal cartilage resorption remains unknown. Thus, our observations may serve as the starting point for future studies in this direction.
keywords:

chondrocyte antigen, cartilage oligomeric matrix protein (COMP), transmembrane p24 trafficking protein 10 (TMP21)

references:
Bacsich P, Wyburn GM (1947): The significance of the mucoprotein content on the survival of homografts of cartilage and cornea. Proc R Soc Edin Sect B 62: 321-327.
Heyner S (1969): The significance of the intercellular matrix in the survival of cartilage allografts. Transplantation 8: 666-677.
Heyner S (1973): The antigenicity of cartilage grafts. Surg Gynec Obstet 135: 298-305.
Otero M, Goldring MB (2007): Cells of the synovium in rheumatoid arthritis Chondrocytes. Arthritis Res Ther 9: 220.
Gao Y, Liu S, Huang J, et al. (2014): The ECM-Cell Interaction of Cartilage Extracellular Matrix on Chondrocytes. Biomed Res Int 2014: 648459.
Krco CJ, Pawelski J, Harders J, et al. (1996): Characterization of the antigenic structure of human type II collagen. J Immunol 156: 2761-2768.
Misják P, Bősze S, Horváti K, et al. (2013): The role of citrullination of an immunodominant proteoglycan (PG) aggrecan T cell epitope in BALB/c mice with PG-induced arthritis. Immunol Lett 152: 25-31.
Moskalewski S, Kawiak J, Rymaszewska T (1966): Local cellular response evoked by cartilage formed after auto- and allogeneic transplantation of isolated chondrocytes. Transplantation 4: 572-581.
Elves MW (1974): A study of the transplantation antigens on chondrocytes from articular cartilage. J Bone Joint Surg 56-B: 178-185.
Malseed ZM, Heyner S (1976): Antigenic profile of the rat chondrocytes. Arthritis Rheum 19: 223-231.
Lance EM (1989): Immunological reactivity towards chondrocytes in rat and man: relevance to autoimmune arthritis. Immunol Lett 21: 63-73.
Lance EM, Kimura LH, Manibog CN (1993): The expression of major histocompatibility antigens on human articular chondrocytes. Clin Orthop Rel Res 291: 266-282.
Bujia J, Alsalameh S, Sittinger M, et al. (1994): Antigen presenting cell function of class II positive human nasal chondrocytes. Acta Otolaryngol 114: 75-79.
Tiku ML, Liu SW, Weaver CW, et al. (1985): Class II histocompatibility antigen-mediated immunologic function of normal articular chondrocytes. J Immunol 135: 2923-2928.
O’Sullivan NA, Ibusuki S, Yaremchuk MJ, Randolph MA (2006): Antigenicity of allogeneic human chondrocytes for tissue engineering. 52nd Annual Meeting of the Orthopaedic Research Society. Paper No: 1446.
Abe S, Nochi H, Ito H (2016): Alloreactivity and immunosuppressive properties of articular chondrocytes from osteoarthritic cartilage. J Orthop Surg (Hong Kong) 24: 232-239.
Lohan P, Treacy O, Lynch K, et al. (2016): Culture expanded primary chondrocytes have potent immunomodulatory properties and do not induce an allogeneic immune response. Osteoarthritis and Cartilage 24: 521-533.
Langer F, Gross AE, Greaves MF (1972): The auto-immunogenicity of articular cartilage. Clin Exp Immunol 12: 31-37.
Gertzbein SD, Lance EM (1976): The stimulation of lymphocytes by chondrocytes in mixed culture. Clin Exp Immunol 24: 102-109.
Gertzbein SD, Tait JH, Devlin SR, Argue S (1977): The antigenicity of chondrocytes. Immunology 33: 141-145.
Glant T, Mikecz K (1986): Antigenic profiles of human, bovine and canine articular chondrocytes. Cell Tissue Res 244: 359-369.
Osiecka-Iwan A, Hyc A, Moskalewski S (1999): Immunosuppression and rejection of cartilage formed by allogeneic chondrocytes in rats. Cell Transpl 8: 627-636.
Osiecka-Iwan A, Hyc A, Jozwiak J, et al. (2003): Transplants of Rat Chondrocytes Evoke Strong Humoral Response Against Chondrocyte-Associated Antigen in Rabbits. Cell Transplant 12: 389-398.
Mayne R, Vail MS, Maine PM, Miller EJ (1976): Changes in type of collagen synthesis as clones of chick chondrocyte grow and eventually lose division capacity. Proc Natl Acad Sci USA 73: 1674-1678.
Schlitz JR, Mayne R, Holtzer H (1973): The synthesis of collagen and glycosaminoglycans by dedifferentiated chondrocytes in culture. Differentiation 1: 97-108.
Osiecka-Iwan A, Hyc A, Niderla-Bielinska J, Moskalewski S (2008): Chondrocyte-associated antigen and matrix components in a 2- and 3-dimensional culture of rat chondrocytes. Mol Med Rep 1: 881-887.
Benya PD, Shaffer JD (1982): Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30: 215-224.
Bonaventure J, Kadhom N, Cohen-Solal L, et al. (1994): Reexpression of cartilage-specific genes by dedifferentiated human articular chondrocytes cultured in alginate beads. Exp Cell Res 212: 97-104.
Osiecka-Iwan A, Niderla-Bielinska J, Hyc A, Moskalewski S (2014): Rat chondrocyte-associated antigen identified as sia­lylated transmembrane protein Tmp21 belonging to the p24 protein family. Calcif Tissue Int 94: 348-352.
Strating JR, van Bakel NH, Leunissen JA, Martens GJ (2009): A comprehensive overview of the vertebrate p24 family: identification of a novel tissue-specifically expressed member. Mol Biol Evol 26: 1707-1714.
Hosaka M, Watanabe T, Yamauchi Y, et al. (2007): A subset of p23 localized on secretory granules in pancreatic beta-cells.
J Histochem Cytochem 55: 235-245.
Füllenkrug J, Suganuma T, Tang BL, et al. (1999): Localization and recycling of gp27 (hp24c3): complex formation with other p24 family members. Mol Biol Cell 10: 1939-1955.
Blum R, Lepier A (2008): The luminal domain of 23 (Tmp21) plays a critical role in p23 cell surface trafficking. Traffic 9: 1520-1550.
Alsalameh S, Mollenhauer J, Hain N, et al. (1990): Cellular immune response towards human articular chondrocytes. Arthrit. Rheumat 33: 1477-1486.
Sabbatini AC, Tacchetti S, Tommasi F, et al. (2003): Use of human chondrocyte cell cultures to identify and characterize reactive antibodies in rheumatoid arthritis sera. Clin Exp Rheumatol 21: 587-592.
Agarwal P, Zwolanek D, Keene DR, et al. (2012): Collagen XII and XIV, new partners of cartilage oligomeric matrix protein in the skin extracellular matrix suprastructure. J Biol Chem 287: 22549-22559.
Wang L, Zheng J, Du Y, et al. (2010): Cartilage oligomeric matrix protein maintains the contractile pheno-type of vascular smooth muscle cells by interacting with alpha(7)beta(1) integrin. Circ Res 106: 514-525.
Müller G, Michel A, Altenburg E (1998): COMP (cartilage oligomeric matrix protein) is synthesized in ligament, tendon, meniscus, and articular cartilage. Connect Tissue Res 39: 233-244.
Acharya C, Yik JH, Kishore A, et al. (2014): Cartilage oligomeric matrix protein and its binding partners in the cartilage extracellular matrix: Interaction, regulation and role in chondrogenesis. Matrix Biology 37: 102-111.
McKee AS, Munks MW, Marrack P (2007): How Do Adjuvants Work? Important Considerations for New Generation Adjuvants. Immunity 27: 687-690.
Kleinau S, Erlandsson H, Holmdahl R, Klareskog L (1991): Adjuvant oils induce arthritis in the DA rat. I. Characterization of the disease and evidence for an immunological involvement. J Autoimmun 4: 871-880.
Rock MJ (2010): Cartilage oligomeric matrix protein promotes cell attachment via two independent mechanisms involving CD47 and V3 integrin. Mol Cell Biochem 338: 215-224.
Koelling S, Clauditz TS, Kaste M, Miosge N (2006): Cartilage oligomeric matrix protein is involved in human limb development and in the pathogenesis of osteoarthritis. Arthritis Res Ther 8: R56.
Mansson B, Carey D, Alini M, et al. (1995): Cartilage and bone metabolism in rheumatoid arthritis. Differences between rapid and slow progression of disease identified by serum markers of cartilage metabolism. J Clin Invest 95: 1071-1077.
DiCesare PE, Carlson CS, Stolerman ES, et al. (1996): Increased degradation and altered tissue distribution of cartilage oligomeric matrix protein in human rheumatoid and osteoarthritic cartilage. J Orthop Res 14: 946-955.
Wisłowska M, Jabłońska B (2005): Serum cartilage oligomeric matrix protein (COMP) in rheumatoid arthritis and knee osteoarthritis. Clin Rheumatol 24: 278-284.
Posey KL, Hecht JT (2008): The role of cartilage oligomeric matrix protein (COMP) in skeletal disease. Curr Drug Targets 9: 869-877.
Williams FM, Spector TD (2008): Biomarkers in osteoarthritis. Arthritis Res Ther 10: 101.
Verma P, Dalal K (2013): Serum cartilage oligomeric matrix protein (COMP) in knee osteoarthritis: a novel diagnostic and prognostic biomarker. J Orthop Res 31: 999-1006.
Weitoft T, Larsson A, Saxne T, et al. (2017): Pentraxin 3 in serum and synovial fluid of patients with rheumatoid arthritis with and without autoantibodies. Scand J Rheumatol 46: 346-352.
Carlsén S, Hansson AS, Olsson H, et al. (1998): Cartilage oligomeric matrix protein (COMP)-induced arthritis in rats. Clin Exp Immunol 114: 477-484.
Molina JF, Espinoza LR (2000): Relapsing polychondritis. Baillieres Best Pract Res Clin Rheumatol 14: 97-109.
Chopra R, Chaudhary N, Kay J (2013): Relapsing polychondritis. Rheum Dis Clin N Am 39: 263-276.
Sharma A, Gnanapandithan K, Sharma K, Sharma S (2013): Relapsing polychondritis: a review. Clin Rheumatol 32: 1575-1583.
Hirayama K, Iwanaga N, Izumi Y, et al. (2016): A Case of Relapsing Polychondritis Initiating with Unexplained Fever. Case Rep Med 2016: 9462489.
Hansson A-S, Holmdahl R (2002): Cartilage-specific autoimmunity in animal models and clinical aspects in patients – focus on relapsing polychondritis. Arthritis Res 4: 296-301.
FEATURED PRODUCTS
Quick links
© 2018 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe