eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank

vol. 43
Clinical immunology

In vitro chromosomal radiosensitivity in patients with common variable immunodeficiency

Majid Mahmoodi, Hassan Abolhassani, Hossein Mozdarani, Nima Rezaei, Gholamreza Azizi, Reza Yazdani, Farideh Farzanfar, Hosein Rafiemanesh, Mohammad-Ali Mohagheghi, Kouros Divsalar, Rasoul Nasiri Kalmarzi, Asghar Ramyar, Asghar Aghamohammadi

(Centr Eur J Immunol 2018; 43 (2): 155-161)
Online publish date: 2018/06/30
View full text
Get citation
JabRef, Mendeley
Papers, Reference Manager, RefWorks, Zotero
Common variable immunodeficiency (CVID) is one of the predominant antibody deficiency disorders, some evidence of which indicates that chromosome instability is present in these patients. An increased risk of cancer in patients with CVID has been documented. This study was undertaken to highlight radiation sensitivity in CVID patients and to clarify the genetic basis of this defect in these cases. Stimulated lymphocytes of the studied subjects were exposed to low-dose gamma-rays in the G2 phase or the G0 phase of the cell cycle and chromosomal aberrations were scored. Lymphocytes of healthy individuals, ataxia telangiectasia (AT) cases and a group of acute lymphoblastic leukemia (ALL) patients were investigated in the same way as controls. By two methods of analysis (one-way ANOVA and unpaired t-test), the CVID cases were significantly more radiosensitive than healthy controls based on the results of the G2 and the G0 assays. First-degree relatives of CVID patients were radiosensitive by the micronucleus assay which showed a significant difference as compared with normal controls (p = 0.001). In conclusion, this study may support that chromosomal radiosensitivity in CVID patients is a marker of genetic predisposition to the disease. The results might be a clue to describe the increased risk of cancer in CVID patients.

primary immunodeficiency, common variable immunodeficiency, chromosome radio­sensitivity, acute lymphoblastic leukemia, ataxia telangiectasia

Chapel H, Cunningham-Rundles C (2009): Update in understanding common variable immunodeficiency disorders (CVIDs) and the management of patients with these conditions. Br J Haematol 145: 709-727.
Chapel H, Lucas M, Lee M, et al. (2008): Common variable immunodeficiency disorders: division into distinct clinical phenotypes. Blood 112: 277-286.
Aghamohammadi A, Farhoudi A, Moin M, et al. (2005): Clinical and immunological features of 65 Iranian patients with common variable immunodeficiency. Clin Diagn Lab Immunol 12: 825-832.
Cunningham-Rundles C, Bodian C (1999): Common variable immunodeficiency: clinical and immunological features of 248 patients. Clin Immunol 92: 34-48.
Hermaszewski RA, Webster AD (1993): Primary hypogammaglobulinaemia: a survey of clinical manifestations and complications. Q J Med 86: 31-42.
Yazdani R, Hakemi MG, Sherkat R, et al. (2014): Genetic defects and the role of helper T-cells in the pathogenesis of common variable immunodeficiency. Adv Biomed Res 9: 2.
Azizi G, Abolhassani H, Asgardoon MH, et al. (2016): Autoimmunity in common variable immunodeficiency: epidemiology, pathophysiology and management. Expert Rev Clin Immunol 16: 1-15.
Pastorelli G, Roncarolo MG, Touraine JL, et al. (1989): Interleukin-4 suppresses immunoglobulin production by peripheral blood lymphocytes of patients with common variable immunodeficiency (CVI) induced by supernatants of T cell clones. Clin Exp Immunol 78: 341-347.
Sneller MC, Strober W (1990): Abnormalities of lymphokine gene expression in patients with common variable immunodeficiency. J Immunol 144: 3762-3769.
Kokron CM, Errante PR, Barros MT, et al. (2004): Clinical and laboratory aspects of common variable immunodeficiency. An Acad Bras Cienc 76: 707-726.
Vorechovsky I, Munzarova M, Lokaj J (1989): Increased bleomycin-induced chromosome damage in lymphocytes of patients with common variable immunodeficiency indicates an involvement of chromosomal instability in their cancer predisposition. Cancer Immunol Immunother 29: 303-306.
Vorechovsky I, Litzman J, Lokaj J, et al. (1990): Common variable immunodeficiency and malignancy: a report of two cases and possible explanation for the association. Cancer Immunol Immunother 31: 250-254.
Ahmad F, Dalvi R, Chavan D, et al. (2008): Cytogenetic profile of acute lymphocytic leukemia patients: report of a novel translocation t(4; 13)(q21 × 3; q35) from an Indian population. Hematology 13: 28-33.
Somasundaram R, Prasad M, Ungerbäck J, et al. (2015): Transcription factor networks in B-cell differentiation link development to acute lymphoid leukemia. Blood 126: 144-152.
Chiaretti S, Foa R (2009): T-cell acute lymphoblastic leukemia. Haematologica 94: 160-162.
Ferrando AA, Look AT (eds.) (2000). Clinical implications of recurring chromosomal and associated molecular abnormalities in acute lymphoblastic leukemia. Seminars in hematology. Elsevier.
Aghamohammadi A, Mohammadinejad P, Abolhassani H, et al. (2014): Primary immunodeficiency disorders in Iran: update and new insights from the third report of the national registry. J Clin Immunol 34: 478-490.
Rezaei N, Aghamohammadi A, Moin M, et al. (2006): Frequency and clinical manifestations of patients with primary immunodeficiency disorders in Iran: update from the Iranian Primary Immunodeficiency Registry. J Clin Immunol 26: 519-532.
Conley ME, Notarangelo LD, Etzioni A (1999): Diagnostic criteria for primary immunodeficiencies. Representing PAGID (Pan-American Group for Immunodeficiency) and ESID (European Society for Immunodeficiencies). Clin Immunol 93: 190-197.
Picard C, Gaspar BH, Al-Herz W, et al. (2018): International Union of Immunological Societies: 2017 Primary Immunodeficiency Diseases Committee Report on Inborn Errors of Immunity. J Clin Immunol 38: 96-128.
West CM, Elyan SA, Berry P, et al. (1995): A comparison of the radiosensitivity of lymphocytes from normal donors, cancer patients, individuals with ataxia-telangiectasia (A-T) and A-T heterozygotes. Int J Radiat Biol 68: 197-203.
Scott D, Barber JB, Spreadborough AR, et al. (1999): Increased chromosomal radiosensitivity in breast cancer patients: a comparison of two assays. Int J Radiat Biol 75: 1-10.
Scott D, Barber JB, Levine EL, et al. (1998): Radiation-induced micronucleus induction in lymphocytes identifies a high frequency of radiosensitive cases among breast cancer patients: a test for predisposition? Br J Cancer 77: 614-620.
Fenech M, Chang WP, Kirsch-Volders M, et al. (2003): HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat Res 534: 65-75.
Gatti RA (2001): The inherited basis of human radiosensitivity. Acta Oncologica 40: 702-711.
Zaki-Dizaji M, Akrami SM, Abolhassani H, et al. (2017): Ataxia telangiectasia syndrome: moonlighting ATM. Expert Rev Clin Immunol 13: 1155-1172.
Aghamohammadi A, Moin M, Kouhi A, et al. (2008): Chromosomal radiosensitivity in patients with common variable immunodeficiency. Immunobiology 213: 447-454.
Vorechovsky I, Scott D, Haeney MR, et al. (1993): Chromosomal radiosensitivity in common variable immune deficiency. Mutat Res 290: 255-264.
Palanduz S, Palanduz A, Yalchin I (1998): In vitro chromosomal radiosensitivity in common variable immunodeficiency. Clin Immunol Immunopathol 86: 180-182.
Offer SM, Pan-Hammarström Q, Hammarström L, et al. (2010): Unique DNA repair gene variations and potential associations with the primary antibody deficiency syndromes IgAD and CVID. PLoS One 5: e12260.
Chua I, Quinti I, Grimbacher B (2008): Lymphoma in common variable immunodeficiency: interplay between immune dysregulation, infection and genetics. Curr Opin Hematol 15: 368-374.
Baeyens A, Thierens H, Claes K, et al. (2002): Chromosomal radiosensitivity in breast cancer patients with a known or putative genetic predisposition. Br J Cancer 87: 1379-1385.
Borgmann K, Haeberle D, Doerk T, et al. (2007): Genetic determination of chromosomal radiosensitivities in G0- and G2-phase human lymphocytes. Radiother Oncol 83: 196-202.
Mozdarani H, Mansouri Z, Haeri SA (2005): Cytogenetic radiosensitivity of g0-lymphocytes of breast and esophageal cancer patients as determined by micronucleus assay. J Radiat Res 46: 111-116.
Holt SM, Georgakilas AG (2007): Detection of complex DNA damage in gamma-irradiated acute lymphoblastic leukemia Pre-b NALM-6 cells. Radiat Res 168: 527-534.
Ramyar A, Aghamohammadi A, Mozdarani H, et al. (2011): Assessment of in vitro chromosomal sensitivity to low doses of gamma irradiation in patients with acute lymphoblastic leukemia. BCCR 2: 2-6.
López-Rocha E, Rodríguez-Mireles K, Segura-Méndez N, et al. (2015): [Malignancies in adult patients with common variable immunodeficiency]. Rev Alerg Mex 62: 22-27.
Sanford KK, Parshad R, Gantt R, et al. (1989): Factors affecting and significance of G2 chromatin radiosensitivity in predisposition to cancer. Int J Radiat Biol 55: 963-981.
Quick links
© 2019 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe