eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank

4/2018
vol. 43
 
Share:
Share:
more
 
 
abstract:
Clinical immunology

Incidence of pituitary autoantibodies in idiopathic diabetes insipidus

Paweł Gut, Agata Czarnywojtek, Katarzyna Ziemnicka, Joanna Waligórska-Stachura, Nadia Sawicka-Gutaj, Maciej Bączyk, Jakub Fischbach, Kosma Woliński, Elżbieta Wrotkowska, Marek Ruchała

(Centr Eur J Immunol 2018; 43 (4): 428-433)
Online publish date: 2018/12/31
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Diabetes insipidus is a disorder resulting from insufficient action of vasopressin (ADH) characterized by excretion of highly diluted urine in large amounts. Idiopathic diabetes insipidus is associated with the presence of both autoantibodies against ADH-secreting neurons and pituitary autoantibodies. The aim of the present study was to evaluate the occurrence of autoantibodies against the pituitary microsomal fraction. The study included 33 sera of diabetes insipidus patients and 10 control sera obtained from 10 healthy persons. In all patients the secretion of pituitary hormones and thyroid autoantibodies was assessed. Human pituitaries were obtained during autopsy and homogenized in 0.01 mol/l pH 7.4 phosphate buffer. In addition, for the autoantibody evaluation, the electrophoretic method of separation in polyacrylamide gel and western blot were employed. Among the 33 subjects, in 23 patients the presence of autoantibodies against the pituitary was shown. Sera of 15 patients reacted with the pituitary microsomal fraction protein of 55 kDa. In other cases, 10 sera reacted with the pituitary antigen of 67 kDa. In addition, 5 sera reacted with the 60 kDa antigen, 5 sera with 52 kDa protein, 3 sera with 105 kDa protein, 3 sera with the 97 kDa antigen and 2 sera with pituitary antigen of 92 kDa weight. In our study, based on the immunoblotting method, we observed that pituitary autoantibodies against 55, 60 and 67 kDa antigens occurred frequently.
keywords:

diabetes insipidus, pituitary autoantibodies, immunoblotting

references:
Komatsu M, Kondo T, Yamauchi K, et al. (1988): Antipituitary antibodies in patients with the primary empty sella syndrome. J Clin Endocrinol Metab 67: 633-638.
Kajita K, Yasuda K, Yamakita N, et al. (1991): Anti-pituitary antibodies in patients with hypopitiutarism and their families: longitudinal observation. Endocrinol Jpn 1991; 38: 121-129.
Kobayashi I, Inukai T, Takahashi M, et al. (1988): Anterior pituitary cell antibodies detected in Hashimoto’s thyroiditis and Grave’s disease. Endocrinol Jpn 35: 705-708.
Kosowicz J, Sawicka J, Gryczyńska M (1994): Pituitary antigens recognised by autoantibodies in endocrinopathies. Endokrynol Pol 45: 139.
Pouplard A, Bottazzo GF, Doniach D, et al. Binding of human immunoglobulins to pituitary ACTH cells. Nature 1976; 261: 142-144.
Mirakian R, Cudworth AG, Bottazzo GF, et al. (1982): Autoimmunity to anterior pituitary cells and the pathogenesis of insulin dependent diabetes mellitus. Lancet 1: 755-759.
Sugiura M, Hashimoto A, Shizawa M, et al. (1986): Heterogenity of anterior pituitary cell antibodies detected in insulin-dependent diabetes mellitus and adrenocorticotropic hormone deficiency. Diabetes Res 3: 111-114.
Nishiyama S, Takano T, Hidaka Y, et al. (1993): A case of postpartum hypopituitarism associated with empty sella: possible relation to postpartum autoimmune hypophysis. Endocr J 40: 431-438.
Bottazzo GF, Doniach D (1978): Pituitary autoimmunity: a review. J Royal Society Med 71: 433-436.
Bottazzo GF, Florin-Christensen A, Pouplard A, et al. (1975): Autoantibodies to prolactin-secreting cells of human pituitary. Lancet 19: 97-101.
Yabe S, Murakami M, Maruyama K, et al. (1995): Western-blot analysis of rat pituitary antigens recognized by human antipituitary antibodies. Endocr J 42: 115-119.
Bando H, Iguchi G, Fukuoka H (2013): The prevalence of IgG4-related hypophysitis in 170 consecutive patients with hypopituitarism and/or central diabetes insipidus and review of the literature. Eur J Endocrinol 170: 161-172.
Nakasone Y, Oguchi K, Sato Y (2015): Rapid conversion of autoimmune hypophysitis to an empty sella with immediate lowering of the serum IgG4 level. Case Report. Neuro Endocrinol Lett 36: 112-114.
Scherbaum WA, Bottazzo GF (1983): Autoantibodies to vasopressin cells in idiopathic diabetes insipidus: evidence for an autoimmune variant. Lancet 1: 897-901.
Pivonello R, De Bellis A, Faggiano A (2003): Central diabetes insipidus and autoimmunity: relationship between the occurrence of antibodies to arginine vasopressin-secreting cells and clinical, immunological, and radiological features in a large cohort of patients with central diabetes insipidus of known and unknown etiology. J Clin Endocrinol Metab 88: 1629-1636.
Maghnie M, Ghirardello S, De Bellis A (2006): Idiopathic central diabetes insipidus in children and young adults is commonly associated with vasopressin-cell antibodies and markers of autoimmunity. Clin Endocrinol (Oxf) 65: 470-478.
De Bellis A, Sinisi AA, Pane E (2012): Involvement of hypothalamus autoimmunity in patients with autoimmune hypopituitarism: role of antibodies to hypothalamic cells. J Clin Endocrinol Metab 97: 3684-3690.
Chiloiro S, Tartaglione T, Angelini F (2017): An overview of diagnosis of primary autoimmune hypophysitis in a single prospective monocentric experience. Neuroendocrinology 104: 280-290.
Falorni A, Minarelli V, Bartoloni E (2014): Diagnosis and classification of autoimmune hypophysitis. Autoimmun Rev 13: 412-416.
Ziemnicka K, Gut P, Gołąb M, et al. (2016): Pituitary microsomal autoantibodies in patients with childhood-onset combined pituitary hormone deficiency: an antigen identification attempt. Arch Immunol Ther Exp (Warsz) 64: 485-495.
Bensing S, Kasperlik-Załuska AA, Czarnocka B, et al. (2005): Autoantibodies against pituitary proteins in patients with adrenocorticotropin-deficiency. Eur J Clin Invest 35: 126-132.
Kasperlik-Załuska AA, Czarnocka B, Czech W (2003): Autoimmunity as the most frquent cause of idiopathic secondary adrenal insufficiency: report of 111 cases. Autoimmunity 36: 155-159.
Crock P, Salvi M, Miller A, et al. (1993): Detection of anti-pituitary autoantibodies by immunoblotting. J Immunol Methods 162: 31-40.
Crock PA (1998): Cytosolic autoantigens in lymphocytic hypophysitis. J Clin Endocrinol Metab 83: 609-618.
Nishiki M, Murakami Y, Ozawa Y, et al. (2001): Serum antibodies to human pituitary membrane antigens in patients with autoimmune lymphocytic hypophysitis and infundibuloneurohypophysitis. Clin Endocrinol 54: 327-333.
Lim S, Elston MS, Swarbrick MJ (2009): Lymphocytic hypophysitis with associated thyroiditis in a man with aseptic meningitis. Pituitary 12: 375-379.
Manetti L, Lupi I, Morselli LL (2007): Prevalence and functional significance of antipituitary antibodies in patients with autoimmune and non-autoimmune thyroid diseases. J Clin Endocrinol Metab 92: 2176-2181.
Garovic VD, Clarke BL, Chilson TS, Specks U (2001): Diabetes insipidus and anterior pituitary insufficiency as presenting features of Wegener’s granulomatosis. Am J Kidney Dis 37: E5.
Kajiyama H, Terai C, De Bellis A (2004): Vasopressin cell antibodies and central diabetes insipidus in a patient with systemic lupus erythematosus and dermatomyositis. J Rheumatol 31: 1218-1221.
Baekkeskov S, Aanstoot HJ, Christgau S, et al. (1990): Identification of the 64K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase. Nature 347: 151-156.
Sawicka J, Gryczyńska M, Baumann-Antczak A, Gut P (1999): Detection of pituitary autoantibody in patients with pituitary tumors and hypopituitarism. Pol Arch Med Wewn 101: 123-129.
Gut P, Kosowicz J, Ziemnicka K (2009): Isolation of pituitary autoantigen by affinity chromatography. Endokrynol Pol 60: 455-460.
Gut P, Fischbach J, Ziemnicka K (2014): Purification of pituitary autoantigen by column liquid chromatography and chromatofocusing. Cent Eur J Immunol 39: 46-50.
FEATURED PRODUCTS
Quick links
© 2019 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe