eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank

vol. 43
Clinical immunology

Is oxidative stress measured by thiol/disulphide homeostasis status associated with prostate adenocarcinoma?

Mehmet Giray Sönmez, Betül Kozanhan, Çigˇdem D. Deniz, Yunus Emre Göger, Muzaffer T. Kılınç, Salim Neşeliogˇlu, Özcan Erel

(Centr Eur Immunol 2018; 43 (2): 174-179)
Online publish date: 2018/06/30
View full text
Get citation
JabRef, Mendeley
Papers, Reference Manager, RefWorks, Zotero
Aim of the study
We aim to examine the relation between thiol/disulphide homeostasis and transrectal ultrasound guided prostate biopsy (TRUS-Bx) results and evaluate whether it was effective on the distinction of benign and malign prostate disease.

Material and methods
The study included 29 men histopathologically diagnosed as prostate adenocancer (Pca) (group 1), 30 men having benign prostate hyperplasia (BPH) (group 2) and age match 30 healthy individuals in the control group (group 3). Thiol/disulphide homeostasis was measured using a novel automatic and spectrophotometric method.

Among the three groups, a statistically significant difference was detected among native thiol, total thiol levels and disulphide/total thiol, disulphide/native thiol and native thiol/total thiol ratios which are thiol/disulphide homeostasis parameters apart from disulphide (p < 0.001, p < 0.001, p < 0.001, p < 0.001, p < 0.001 respectively). Additionally, no significant difference was detected in albumin and total protein levels among the groups (p = 0.223, p = 0.316 respectively). Serum native and total thiol levels were high and disulphide level was low in group 1 when compared to the group 2 (p = 0.003, p = 0.007, p = 0.265 respectively). In addition, serum native thiol, total thiol and disulphide levels were low in group 1 when compared to the group 3, but while low native and total thiol levels were significant, low disulphide levels were not found significant (p < 0.001, p < 0.001, p = 0.331, respectively).

Thiol/disulphide homeostasis was found to be disturbed in Pca patients detected with TRUS-Bx. This is suggesting serum native thiol, total thiol level and ratios provides a novel biomarker for the role for oxidative stress in disease etiopathogenesis.


dynamic thiol-disulphide homeostasis, prostate cancer, benign prostatic hyperplasia, oxidative stress

Mottet N, Bellmunt J, Bolla M, et al. (2017): EAU-ESTRO-SIOG Guidelines on Prostate Cancer. Part 1: Screening, Diagnosis, and Local Treatment with Curative Intent.Eur Urol 71: 618-629.
Cabarkapa S, Perera M, McGrath S, Lawrentschuk N. (2016 ): Prostate cancer screening with prostate-specific antigen: A guide to the guidelines. Prostate Int Dec 4: 125-129.
Çömez K, Bozkurt O, Mungan MU (2015): Prostate Biopsy 2014: TRUS, MRI/TRUS Fusion? Transrectal? Transperineal? Bulletin of Urooncology 14: 128-135.
Engin A, Altan N (2000): Effects of obstructive jaundice on the antioxidative capacity of human red blood cells. Hematologia 30: 91-6.
YardIm-AkaydIn S, Sepici A, Özkan Y, Torun M, Şimşek B, Sepici V (2004): Oxidation of Uric Acid in Rheumatoid Arthritis: Is Allontoin a Marker of Oxidative Stress? Free Radic Res 38: 623-628.
Jackson AL, Loeb LA (2001): The contribution of endogenous sources of DNA damage to the multiple mutations in cancer. Mutat Res 477: 7-21.
Behrend L, Henderson G, Zwacka RM (2003): Reactive oxygen species in oncogenic transformation. Biochem Soc Trans 31: 1441-1444.
Orhan N, Özcan ME, MemişogˇullarI R, Uçgun T, KayIkçI MA, Demirin H (2015): Oxidative Stress and Decrease of Paroxonase Activity in Patients whit Prostate Cancer. Konuralp TIp Dergisi 7: 113-117
Udensi UK, Tchounwou PB (2016): Oxidative stress in prostate hyperplasia and carcinogenesis. J Exp Clin Cancer Res Sep 8; 35: 139.
Erel O, Neselioglu S (2014): A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 47: 326-332.
AltIparmak IH, Erkuş ME, Sezen H, et al. (2016): The relation of serum thiol levels and thiol/disulfide homeostasis with the severity of coronary artery disease.Kardiol Pol 74: 1346-1353.
Ates I, Kaplan M, Yuksel M, et al. (2016): Determination of thiol/disulphide homeostasis in type 1 diabetes mellitus and the factors associated with thiol oxidation. Endocrine 51: 47-51.
Dirican N, Dirican A, Sen O, et al. (2016): Thiol/disulfide homeostasis: A prognostic biomarker for patients with advanced non-small celllung cancer? Redox Rep 21: 197-203.
Bal C, Büyükşekerci M, Koca C, et al. (2016): The compromise of dynamic disulphide/thiol homeostasis as a biomarker of oxidative stres in trichloroethylene exposure. Human and Experimental Toxicology 35: 915-920.
Bostwick DG, Burke HB, Djakiew D, et al. (2004): Human prostate cancer risk factors. Cancer 101 (Suppl): 2371-2490.
Khandrika L, Kumar B, Koul S, Maroni P, Koul HK (2009): Oxidative stress in prostate cancer. Cancer Lett 282: 125-136.
Gupta-Elera G, Garrett AR, Robison RA, O’Neill KL (2012): The role of oxidative stress in prostate cancer. Eur J Cancer Prev 21: 155-162.
Paschos A, Pandya R, Duivenvoorden WCM, Pinthus JH (2013): Oxidative Stress in Prostate Cancer. Prostate Cancer Prostatic Dis 16: 217-225.
Droge W (2002): Free radicals in the physiological control of cell function. Physiol Rev 82: 47-95.
Dalle-Donne I, Rossi R, Colombo R, Giustarini D, Milzani A (2006): Biomarkers of oxidative damage in human disease. Clin Chem 52: 601-623.
Reznick AZ, Packer L (1994): Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 233: 357-363.
Levine RL, Williams JA, Stadtman ER, et al. (1994): Carbonyl assays for determination of oxidatively modified proteins. Methods Enzymol 233: 347-357.
Hu ML (1994): Measurement of protein thiol groups and glutathione in plasma. Methods Enzymol 233: 380-385.
Demirseren DD, Cicek C, Alisik M, Demirseren ME, Aktaş A, Erel O (2017): Dynamic thiol/disulphide homeostasis in patients with basal cell carcinoma.Cutan Ocul Toxicol 1: 1-5; doi: 10.1080/15569527.2016.1268150.
Hanikoglu F, Hanikoglu A, Kucuksayan E, et al. (2016): Dynamic thiol/disulphide homeostasis before and after radical prostatectomy in patients with prostate cancer. Free Radic Res 50 (suppl 1): 79-84.
Yazici O, Dogan M, Erel O, et al. (2015): The importance of thiol/disulphide homeostasis in patients with advanced colorectal cancer receiving modified fluorouracil and leucoverin plus oxaliplatin (mFOLFOX) regimen. Eur J Cancer 51 (Suppl 3): 399.
Guney T, Kanat IL, Alkan A, et al. (2016): Assessment of serum thiol/disulfide homeostasis in multiple myeloma patients by a new method. Redox Rep 19: 1-6; doi: 10.1080/13510002.2016.1180100.
Quick links
© 2019 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe