eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank

3/2018
vol. 43
 
Share:
Share:
more
 
 
abstract:
Clinical immunology

Lymphocyte subgroups and recurrent infections in children with Down syndrome – a prospective case control study

Maha Mitwalli, Yahya Wahba, Ali Shaltout, Mona Gouida

(Centr Eur J Immunol 2018; 43 (3): 248-254)
Online publish date: 2018/10/30
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
Down syndrome (DS) is the commonest genetic disorder and more liable for recurrent infections. We aimed to determine the differences in lymphocyte subgroups between DS children and the healthy population and to study the pattern and likelihood for recurrent infections and hospital admission due to infection. Our study was carried out in the Genetic Unit of Mansoura University Children’s Hospital, Egypt. The study enrolled 150 DS (DS group) and 100 controls (CG group). They were assessed for recurrent infections (including tonsillitis, otitis media [OM], pneumonia, upper respiratory tract infections [URTI], sinusitis, and gastroenteritis [GE]) and hospital admission due to infections. All patients were subjected to complete blood count and flow cytometric analysis for expression markers of B lymphocytes (CD19), natural killer (NK) cells (CD56), and T lymphocytes (CD3, CD4 and CD8). We found a statistically significant increase in the frequency of URTIs and sinusitis, OM, pneumonia, and hospital admission in the DS group. As regards the type of recurrent infection in DS, it was highest for URTIs and sinusitis. For age groups below 13 years, a statistically significant decrease in all studied CD markers was found in the DS group, while for the 13-18-year-olds, a statistically significant decrease was found in CD4, CD19, and CD56 in the DS group. Non-significant correlations were found between CD markers and recurrent infection and hospital admission. We concluded that lymphocyte subgroups that carry CD3, CD4, CD8, CD19, and CD56 were decreased in DS. Recurrent infections and hospital admission are still striking feature for DS but are not significantly correlated with lymphocyte subgroups.
keywords:

Down syndrome, lymphocyte subgroups, recurrent infections, CD markers

references:
Morris JK, Springett A (2014): The National Down Syndrome Cytogenetic Register for England and Wales: 2010 Annual Report. London Public Heal Engl.
Kusters MAA, Verstegen RHJ, Gemen EFA, De Vries E (2009): Intrinsic defect of the immune system in children with Down syndrome: a review. Clin Exp Immunol 156: 189-193.
McDowell KM, Craven DI (2011): Pulmonary complications of Down syndrome during childhood. J Pediatr 158: 319-325.
Cruz N V, Mahmoud SA, Chen H, et al. (2009): Follow-up study of immune defects in patients with dysmorphic disorders. Ann Allergy Asthma Immunol 102: 426-431.
Ram G, Chinen J (2011): Infections and immunodeficiency in Down syndrome. Clin Exp Immunol 164: 9-16.
Kusters MAA, Gemen EFA, Verstegen RHJ, et al. (2010): Both normal memory counts and decreased naive cells favor intrinsic defect over early senescence of Down syndrome T lymphocytes. Pediatr Res 67: 557-562.
Lott IT (1982): Down’s syndrome, aging, and Alzheimer’s disease: a clinical review. Ann N Y Acad Sci 396: 15-27.
Cuadrado E, Barrena MJ (1996): Immune dysfunction in Down’s syndrome: primary immune deficiency or early senescence of the immune system? Clin Immunol Immunopathol 78: 209-214.
Holmes DK, Bates N, Murray M, et al. (2006): Hematopoietic progenitor cell deficiency in fetuses and children affected by Down’s syndrome. Exp Hematol 34: 1611-1615.
Megged O, Schlesinger Y (2010): Down syndrome and respiratory syncytial virus infection. Pediatr Infect Dis J 29: 672-673.
Watts R, Vyas H (2013): An overview of respiratory problems in children with Down’s syndrome. Arch Dis Child 98: 812-817.
De Hingh YC, Van Der Vossen PW, Gemen EFA, et al. (2005): Intrinsic abnormalities of lymphocyte counts in children with down syndrome. J Pediatr 147: 744-747.
Zizka Z, Calda P, Fait T, et al. (2006): Prenatally diagnosable differences in the cellular immunity of fetuses with Down’s and Edwards’ syndrome. Fetal Diagn Ther 21: 510-514.
Ghosh S, Feingold E, Dey SK (2009): Etiology of Down syndrome: Evidence for consistent association among altered meiotic recombination, nondisjunction, and maternal age across populations. Am J Med Genet Part A 149: 1415-1420.
Morris JK, Mutton DE, Alberman E (2005): Recurrences of free trisomy 21: analysis of data from the National Down Syndrome Cytogenetic Register. Prenat Diagn 25: 1120-1128.
Ribeiro L, Jacob C, Pastorino AC, et al.(2003): Evaluation factors associated in recurrent and/or severe infections in patients with Down’s syndrome. J Pediatr (Rio J) 79: 141-148.
AlKhater SA (2009): Approach to the child with recurrent infections. J Fam Community Med 16: 77.
Perry S, De la Luz Sanchez M, Hurst PK, Parsonnet J (2005): Household transmission of gastroenteritis. Emerg Infect Dis 11: 1093.
Bossuyt X, Marti GE, Fleisher TA (1997): Comparative analysis of whole blood lysis methods for flow cytometry. Cytometry 30: 124-133.
Jalla S, Sazawal S, Deb S, et al. (2004): Enumeration of lymphocyte subsets using flow cytometry: Effect of storage before and after staining in a developing country setting. Indian J Clin Biochem 19: 95-99.
Seckin AN, Ozdemir H, Ceylan A, Artac H (2017): Age-related alterations of the CD19 complex and memory B cells in children with Down syndrome. Clin Exp Med 18: 125-131.
De Vries E (2006): Patient centred-screening for primary immunodeficiency: a multi-stage diagnostic protocol designed for non-immunologists. Clin Exp Immunol 145: 204-214.
Broers CJM, Gemke RJBJ, Weijerman ME, et al. (2012): Frequency of lower respiratory tract infections in relation to adaptive immunity in children with Down syndrome compared to their healthy siblings. Acta Paediatr 101: 862-867.
Verstegen RHJ, Gameren-Oosterom HBM, Fekkes M, et al. (2013): Significant impact of recurrent respiratory tract infections in children with Down syndrome. Child Care Health Dev 39: 801-809.
Bloemers BLP, van Bleek GM, Kimpen JLL, Bont L (2010): Distinct abnormalities in the innate immune system of children with Down syndrome. J Pediatr 156: 804-809.
Cossarizza A, Monti D, Montagnani G, et al. (1990): Precocious aging of the immune system in Down syndrome: Alteration of β lymphocytes, T-lymphocyte subsets, and cells with natural killer markers. Am J Med Genet Part A 37(S7): 213-218.
Cocchi G, Mastrocola M, Capelli M, et al. (2007): Immunological patterns in young children with Down syndrome: is there a temporal trend? Acta Paediatr 96: 1479-1482.
Zampieri BL, Biselli-Périco JM, de Souza JES, et al. (2014): Altered expression of immune-related genes in children with Down syndrome. PLoS One 9: e107218.
Faddan NA, Sayed D, Ghaleb F (2011): T lymphocytes apoptosis and mitochondrial membrane potential in Down’s syndrome. Fetal Pediatr Pathol 30: 45-52.
Yılmaz C, Dogan M, Başarslan F, et al. (2015): Evaluation of Lymphocyte Subgroups in Children With Down Syndrome. Clin Appl Thromb 21: 546-549.
Cetiner S, Demirhan O, Inal TC, et al. (2010): Analysis of peripheral blood T-cell subsets, natural killer cells and serum levels of cytokines in children with Down syndrome. Int J Immunogenet 37: 233-237.
Verstegen RHJ, Driessen GJ, Bartol SJW, et al. (2014): Defective B-cell memory in patients with Down syndrome. J Allergy Clin Immunol 134: 1346-1353.
Verstegen RHJ, Kusters MAA, Gemen EFA, De Vries E (2010): Down syndrome B-lymphocyte subpopulations, intrinsic defect or decreased T-lymphocyte help. Pediatr Res 67: 563-569.
Ugazio AG, Maccario R, Notarangelo LD, Burgio GR (1990): Immunology of Down syndrome: a review. Am J Med Genet Part A 37(S7): 204-212.
Tolmie JL (1996): Down syndrome and other autosomal trisomies. In: Rimoin DL, Connor JM, Pyeritz RE, Korf BR (eds.). Emery and Rimoin’s Principles and Practice of Medical Genetics (3rd ed.). Churchill Livingstone, New York; 925-971.
FEATURED PRODUCTS
Quick links
© 2018 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe