This study aimed to investigate the impact of an anterior cruciate ligament rupture on the level of competition and match running performance over the following three seasons after return-to-play in professional male football players. Fifty-one football players from LaLiga who sustained a complete anterior cruciate ligament rupture were retrospectively followed over the three seasons after their return-to-play. Their level of competition and match running performance metrics were obtained via Mediacoach® and subsequently compared across different time points: (1) PRE (season before injury); (2) INJ (season of the anterior cruciate ligament rupture); and (3) POST1, (4) POST2, and (5) POST3 (first, second, and third seasons after returning-to-play). Outcomes were analysed overall, by field position and by age group (≤ 25 and > 25 years). By the POST3 season following the anterior cruciate ligament injury, 35 of the 51 players (68.5%) were still competing in one of the top five UEFA leagues (34 in LaLiga and 1 in another top-five league), 11 (21.6%) were playing in lower-tier leagues, and 5 (9.8%) had retired. Maximum running speed decreased at POST1 and POST2 (p < 0.050) compared with PRE. Only players > 25 years experienced a significant decrease in their maximum running speed during POST2 and POST3 (p < 0.050) in comparison with PRE-values. A complete anterior cruciate ligament rupture in football from LaLiga players led to a decline in the level of competition and reductions in their maximum running speed up to three seasons after return-to-play. Players > 25 years were more vulnerable to sustain performance losses.