eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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SCImago Journal & Country Rank
2/2020
vol. 45
 
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abstract:
Clinical immunology

Molecular methods in diagnostics of post-BCG vaccine adverse events

Sylwia Agata Brzezińska
1
,
Teresa Bielecka
2
,
Anna Zabost
1
,
Agnieszka Głogowska
1
,
Monika Kozińska
1
,
Ewa Augustynowicz-Kopeć
1

1.
Department of Microbiology, National Tuberculosis and Lung Diseases Research Institute, Warsaw, Poland
2.
Department of Pediatric Pulmonology and Allergy, Medical University of Warsaw, Warsaw, Poland
Cent Eur J Immunol 2020; 45 (2): 130-135
Online publish date: 2020/07/27
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Introduction
Bacillus Calmette-Guérin (BCG) is the only tuberculosis vaccine available and although it has been routinely used for more than 80 years, its protective effect varies depending on the age and the form of tuberculosis. Due to the close analogy between the vaccine strain and other species of the Mycobacterium tuberculosis complex, molecular methods are recommended for differential diagnosis of post-BCG complications. The aim of the study was to assess usefulness of molecular methods in diagnosis of post-BCG vaccine adverse events (VAEs).

Material and methods
M. tuberculosis complex strains obtained in 2011-2017 from 68 ill children were subjected to molecular analysis.

Results
Molecular analysis of 68 strains showed 100% agreement between the results in the GenoType MTBC method and the multiplex PCR method. For the strains isolated from 45 patients with suspected VAE, M. bovis BCG was obtained, whereas the strains isolated from the remaining 23 children were identified as M. tuberculosis. The analysis confirmed the close relationship between the result of identification and the type of material as well as the patient’s age.

Conclusions
The use of genetic methods enables quick and detailed diagnostics of infections caused by M. bovis BCG, which allows for the confirmation or exclusion of VAE.

keywords:

Bacillus Calmette-Guerin, Mycobacterium tuberculosis, vaccines

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